Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p - PubMed (original) (raw)
Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p
Yonggang Wang et al. Biochem Biophys Res Commun. 2005.
Abstract
A Gtr1p GTPase, the GDP mutant of which suppresses both temperature-sensitive mutants of Saccharomyces cerevisiae RanGEF/Prp20p and RanGAP/Rna1p, was presently found to interact with Yrb2p, the S. cerevisiae homologue of mammalian Ran-binding protein 3. Gtr1p bound the Ran-binding domain of Yrb2p. In contrast, Gtr2p, a partner of Gtr1p, did not bind Yrb2p, although it bound Gtr1p. A triple mutant: yrb2delta gtr1delta gtr2delta was lethal, while a double mutant: gtr1delta gtr2delta survived well, indicating that Yrb2p protected cells from the killing effect of gtr1delta gtr2delta. Recombinant Gtr1p and Gtr2p were purified as a complex from Escherichia coli. The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p.
Similar articles
- Genetic evidence that Ras-like GTPases, Gtr1p, and Gtr2p, are involved in epigenetic control of gene expression in Saccharomyces cerevisiae.
Sekiguchi T, Hayashi N, Wang Y, Kobayashi H. Sekiguchi T, et al. Biochem Biophys Res Commun. 2008 Apr 11;368(3):748-54. doi: 10.1016/j.bbrc.2008.01.133. Epub 2008 Feb 5. Biochem Biophys Res Commun. 2008. PMID: 18258182 - Gtr1p differentially associates with Gtr2p and Ego1p.
Wang Y, Kurihara Y, Sato T, Toh H, Kobayashi H, Sekiguchi T. Wang Y, et al. Gene. 2009 May 15;437(1-2):32-8. doi: 10.1016/j.gene.2009.01.018. Gene. 2009. PMID: 19374031 - A new role of ran GTPase.
Nishimoto T. Nishimoto T. Biochem Biophys Res Commun. 1999 Sep 7;262(3):571-4. doi: 10.1006/bbrc.1999.1252. Biochem Biophys Res Commun. 1999. PMID: 10471364 Review. - [The function of Ran GTPase cycle].
Sekiguchi T, Nishitani H, Nishimoto T. Sekiguchi T, et al. Tanpakushitsu Kakusan Koso. 1999 Sep;44(12 Suppl):1869-76. Tanpakushitsu Kakusan Koso. 1999. PMID: 10503025 Review. Japanese. No abstract available.
Cited by
- Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability.
Shen K, Choe A, Sabatini DM. Shen K, et al. Mol Cell. 2017 Nov 2;68(3):552-565.e8. doi: 10.1016/j.molcel.2017.09.026. Epub 2017 Oct 19. Mol Cell. 2017. PMID: 29056322 Free PMC article. - Unsolved mysteries of Rag GTPase signaling in yeast.
Hatakeyama R, De Virgilio C. Hatakeyama R, et al. Small GTPases. 2016 Oct;7(4):239-246. doi: 10.1080/21541248.2016.1211070. Epub 2016 Jul 11. Small GTPases. 2016. PMID: 27400376 Free PMC article. - The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae.
Sengottaiyan P, Spetea C, Lagerstedt JO, Samyn D, Andersson M, Ruiz-Pavón L, Persson BL. Sengottaiyan P, et al. BMC Biochem. 2012 Jun 24;13:11. doi: 10.1186/1471-2091-13-11. BMC Biochem. 2012. PMID: 22726655 Free PMC article. - Key factors in mTOR regulation.
Bai X, Jiang Y. Bai X, et al. Cell Mol Life Sci. 2010 Jan;67(2):239-53. doi: 10.1007/s00018-009-0163-7. Epub 2009 Oct 13. Cell Mol Life Sci. 2010. PMID: 19823764 Free PMC article. Review. - mTOR signaling: RAG GTPases transmit the amino acid signal.
Shaw RJ. Shaw RJ. Trends Biochem Sci. 2008 Dec;33(12):565-8. doi: 10.1016/j.tibs.2008.09.005. Epub 2008 Oct 15. Trends Biochem Sci. 2008. PMID: 18929489 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous