ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli - PubMed (original) (raw)
ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli
I R Tsaneva et al. Cell. 1992.
Abstract
The RuvA and RuvB proteins of E. coli, which are induced as part of the cellular response to DNA damage, act together to promote the branch migration of Holliday junctions. Addition of purified RuvA and RuvB to a RecA-mediated recombination reaction stimulates the rate of strand exchange and the formation of hetero-duplex DNA. Stimulation does not occur via interaction with RecA; instead, RuvA and RuvB act directly upon recombination intermediates (Holliday junctions) made by RecA. We show that RuvAB-mediated branch migration requires ATP and can bypass UV-induced DNA lesions. At high RuvB concentrations, the requirement for RuvA is overcome, indicating that the RuvB ATPase provides the motor force for branch migration. RuvA protein provides specificity by binding to the Holliday junction, thereby reducing the requirement for RuvB by 50-fold. The newly discovered biochemical properties of RuvA, RuvB, and RuvC are incorporated into a model for the postreplicational repair of DNA following UV irradiation.
Similar articles
- Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.
Iwasaki H, Takahagi M, Nakata A, Shinagawa H. Iwasaki H, et al. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. Genes Dev. 1992. PMID: 1427081 - Interaction of Escherichia coli RuvA and RuvB proteins with synthetic Holliday junctions.
Parsons CA, Tsaneva I, Lloyd RG, West SC. Parsons CA, et al. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5452-6. doi: 10.1073/pnas.89.12.5452. Proc Natl Acad Sci U S A. 1992. PMID: 1608954 Free PMC article. - Biological roles of the Escherichia coli RuvA, RuvB and RuvC proteins revealed.
West SC, Connolly B. West SC, et al. Mol Microbiol. 1992 Oct;6(19):2755-9. doi: 10.1111/j.1365-2958.1992.tb01454.x. Mol Microbiol. 1992. PMID: 1435254 Review. - Formation of a RuvAB-Holliday junction complex in vitro.
Parsons CA, West SC. Parsons CA, et al. J Mol Biol. 1993 Jul 20;232(2):397-405. doi: 10.1006/jmbi.1993.1399. J Mol Biol. 1993. PMID: 8393934 - Processing of Holliday junctions by the Escherichia coli RuvA, RuvB, RuvC and RecG proteins.
Müller B, West SC. Müller B, et al. Experientia. 1994 Mar 15;50(3):216-22. doi: 10.1007/BF01924004. Experientia. 1994. PMID: 8143795 Review.
Cited by
- Generation and Repair of Postreplication Gaps in Escherichia coli.
Cox MM, Goodman MF, Keck JL, van Oijen A, Lovett ST, Robinson A. Cox MM, et al. Microbiol Mol Biol Rev. 2023 Jun 28;87(2):e0007822. doi: 10.1128/mmbr.00078-22. Epub 2023 May 22. Microbiol Mol Biol Rev. 2023. PMID: 37212693 Free PMC article. Review. - Mechanism of AAA+ ATPase-mediated RuvAB-Holliday junction branch migration.
Wald J, Fahrenkamp D, Goessweiner-Mohr N, Lugmayr W, Ciccarelli L, Vesper O, Marlovits TC. Wald J, et al. Nature. 2022 Sep;609(7927):630-639. doi: 10.1038/s41586-022-05121-1. Epub 2022 Aug 24. Nature. 2022. PMID: 36002576 Free PMC article. - RadD is a RecA-dependent accessory protein that accelerates DNA strand exchange.
Bonde NJ, Romero ZJ, Chitteni-Pattu S, Cox MM. Bonde NJ, et al. Nucleic Acids Res. 2022 Feb 28;50(4):2201-2210. doi: 10.1093/nar/gkac041. Nucleic Acids Res. 2022. PMID: 35150260 Free PMC article. - Bifidobacterium animalis: the missing link for the cancer-preventive effect of Gynostemma pentaphyllum.
Liao W, Khan I, Huang G, Chen S, Liu L, Leong WK, Li XA, Wu J, Wendy Hsiao WL. Liao W, et al. Gut Microbes. 2021 Jan-Dec;13(1):1847629. doi: 10.1080/19490976.2020.1847629. Epub 2020 Nov 24. Gut Microbes. 2021. PMID: 33228450 Free PMC article. - Genome maintenance functions of the INO80 chromatin remodeller.
Morrison AJ. Morrison AJ. Philos Trans R Soc Lond B Biol Sci. 2017 Oct 5;372(1731):20160289. doi: 10.1098/rstb.2016.0289. Philos Trans R Soc Lond B Biol Sci. 2017. PMID: 28847826 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases