Isolation and characterization of a novel cardiac adenylylcyclase cDNA - PubMed (original) (raw)
. 1992 Jul 5;267(19):13553-7.
Affiliations
- PMID: 1618857
Free article
Isolation and characterization of a novel cardiac adenylylcyclase cDNA
Y Ishikawa et al. J Biol Chem. 1992.
Free article
Abstract
A novel adenylylcyclase cDNA (type V) was isolated from a canine heart cDNA library. Northern blotting indicates that the expression of this message is most abundant in heart with a lesser amount in brain but is absent in a variety of other tissues including lung, kidney, skeletal muscle, lymphocyte, and testis. The putative protein product predicted from the cDNA sequence has the motif of tandem six-transmembrane spans separated by a large hydrophilic cytoplasmic loop as seen in other members of the adenylylcyclase family. When this protein is expressed using a CMT cell transient expression system, the adenylylcyclase activity was stimulated by NaF, GTP gamma S, and forskolin, but not by calmodulin. The activity was inhibited in a concentration-dependent manner with either P-site active agents such as adenosine or in the presence of calcium. These data indicate that the protein encoded by this cDNA is adenylylcyclase with the biochemical features characteristic of the cardiac isoform.
Similar articles
- Expression and characterization of calmodulin-activated (type I) adenylylcyclase.
Tang WJ, Krupinski J, Gilman AG. Tang WJ, et al. J Biol Chem. 1991 May 5;266(13):8595-603. J Biol Chem. 1991. PMID: 2022671 - In vivo generation of an adenylylcyclase isoform with a half-molecule motif.
Katsushika S, Kawabe J, Homcy CJ, Ishikawa Y. Katsushika S, et al. J Biol Chem. 1993 Feb 5;268(4):2273-6. J Biol Chem. 1993. PMID: 8428899 - Stimulation and inhibition of human platelet adenylylcyclase by thiophosphorylated transducin beta gamma-subunits.
Wieland T, Ronzani M, Jakobs KH. Wieland T, et al. J Biol Chem. 1992 Oct 15;267(29):20791-7. J Biol Chem. 1992. PMID: 1400395 - Volume overload cardiac hypertrophy exhibits decreased expression of g(s)alpha and not of g(i)alpha in heart.
Di Fusco F, Hashim S, Anand-Srivastava MB. Di Fusco F, et al. Am J Physiol Cell Physiol. 2000 Oct;279(4):C990-8. doi: 10.1152/ajpcell.2000.279.4.C990. Am J Physiol Cell Physiol. 2000. PMID: 11003579 - Cloning and characterization of a sixth adenylyl cyclase isoform: types V and VI constitute a subgroup within the mammalian adenylyl cyclase family.
Katsushika S, Chen L, Kawabe J, Nilakantan R, Halnon NJ, Homcy CJ, Ishikawa Y. Katsushika S, et al. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8774-8. doi: 10.1073/pnas.89.18.8774. Proc Natl Acad Sci U S A. 1992. PMID: 1528892 Free PMC article.
Cited by
- Oral angiotensin-converting enzyme inhibitor captopril protects the heart from Porphyromonas gingivalis LPS-induced cardiac dysfunction in mice.
Kiyomoto K, Matsuo I, Suita K, Ohnuki Y, Ishikawa M, Ito A, Mototani Y, Tsunoda M, Morii A, Nariyama M, Hayakawa Y, Amitani Y, Gomi K, Okumura S. Kiyomoto K, et al. PLoS One. 2023 Nov 20;18(11):e0292624. doi: 10.1371/journal.pone.0292624. eCollection 2023. PLoS One. 2023. PMID: 37983238 Free PMC article. - Vidarabine, an anti-herpes agent, improves Porphyromonas gingivalis lipopolysaccharide-induced cardiac dysfunction in mice.
Tsunoda M, Matsuo I, Ohnuki Y, Suita K, Ishikawa M, Mitsubayashi T, Ito A, Mototani Y, Kiyomoto K, Morii A, Nariyama M, Hayakawa Y, Gomi K, Okumura S. Tsunoda M, et al. J Physiol Sci. 2023 Aug 9;73(1):18. doi: 10.1186/s12576-023-00873-5. J Physiol Sci. 2023. PMID: 37558983 Free PMC article. - The role of the type 7 adenylyl cyclase isoform in alcohol use disorder and depression.
Tabakoff B, Hoffman PL. Tabakoff B, et al. Front Pharmacol. 2022 Oct 28;13:1012013. doi: 10.3389/fphar.2022.1012013. eCollection 2022. Front Pharmacol. 2022. PMID: 36386206 Free PMC article. Review. - Vidarabine, an anti-herpes agent, prevents occlusal-disharmony-induced cardiac dysfunction in mice.
Hayakawa Y, Suita K, Ohnuki Y, Mototani Y, Ishikawa M, Ito A, Nariyama M, Morii A, Kiyomoto K, Tsunoda M, Matsuo I, Kawahara H, Okumura S. Hayakawa Y, et al. J Physiol Sci. 2022 Feb 11;72(1):2. doi: 10.1186/s12576-022-00826-4. J Physiol Sci. 2022. PMID: 35148678 Free PMC article. - Dual Activation of Phosphodiesterase 3 and 4 Regulates Basal Cardiac Pacemaker Function and Beyond.
Vinogradova TM, Lakatta EG. Vinogradova TM, et al. Int J Mol Sci. 2021 Aug 5;22(16):8414. doi: 10.3390/ijms22168414. Int J Mol Sci. 2021. PMID: 34445119 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases