Visualization of embryonic neural stem cells using Hes promoters in transgenic mice - PubMed (original) (raw)
Visualization of embryonic neural stem cells using Hes promoters in transgenic mice
Toshiyuki Ohtsuka et al. Mol Cell Neurosci. 2006 Jan.
Abstract
In the central nervous system, neural stem cells proliferate in the ventricular zone (VZ) and sequentially give rise to both neurons and glial cells in a temporally and spatially regulated manner, suggesting that stem cells may differ from one another in different brain regions and at different developmental stages. For the purpose of marking and purifying neural stem cells to ascertain whether such differences exist, we generated transgenic mice using promoters from Hes genes (pHes1 or pHes5) to drive expression of destabilized enhanced green fluorescent protein. In the developing brains of these transgenic mice, GFP expression was restricted to undifferentiated cells in the VZ, which could asymmetrically produce a Numb-positive neuronal daughter and a GFP-positive progenitor cell in clonal culture, indicating that they retain the capacity to self-renew. Our results suggest that pHes-EGFP transgenic mice can be used to explore similarities and differences among neural stem cells during development.
Similar articles
- Identification of self-replicating multipotent progenitors in the embryonic nervous system by high Notch activity and Hes5 expression.
Basak O, Taylor V. Basak O, et al. Eur J Neurosci. 2007 Feb;25(4):1006-22. doi: 10.1111/j.1460-9568.2007.05370.x. Eur J Neurosci. 2007. PMID: 17331197 - Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation.
Hatakeyama J, Bessho Y, Katoh K, Ookawara S, Fujioka M, Guillemot F, Kageyama R. Hatakeyama J, et al. Development. 2004 Nov;131(22):5539-50. doi: 10.1242/dev.01436. Epub 2004 Oct 20. Development. 2004. PMID: 15496443 - Roles of Hes genes in neural development.
Kageyama R, Ohtsuka T, Kobayashi T. Kageyama R, et al. Dev Growth Differ. 2008 Jun;50 Suppl 1:S97-103. doi: 10.1111/j.1440-169X.2008.00993.x. Epub 2008 Apr 22. Dev Growth Differ. 2008. PMID: 18430159 Review. - [Transcription factor network that regulates neural development].
Kageyama R. Kageyama R. Brain Nerve. 2008 Apr;60(4):329-33. Brain Nerve. 2008. PMID: 18421974 Review. Japanese.
Cited by
- Truncated radial glia as a common precursor in the late corticogenesis of gyrencephalic mammals.
Bilgic M, Wu Q, Suetsugu T, Shitamukai A, Tsunekawa Y, Shimogori T, Kadota M, Nishimura O, Kuraku S, Kiyonari H, Matsuzaki F. Bilgic M, et al. Elife. 2023 Nov 21;12:RP91406. doi: 10.7554/eLife.91406. Elife. 2023. PMID: 37988289 Free PMC article. - Reduced chromatin accessibility correlates with resistance to Notch activation.
van den Ameele J, Krautz R, Cheetham SW, Donovan APA, Llorà-Batlle O, Yakob R, Brand AH. van den Ameele J, et al. Nat Commun. 2022 Apr 25;13(1):2210. doi: 10.1038/s41467-022-29834-z. Nat Commun. 2022. PMID: 35468895 Free PMC article. - Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells.
Wu Q, Shichino Y, Abe T, Suetsugu T, Omori A, Kiyonari H, Iwasaki S, Matsuzaki F. Wu Q, et al. Nat Commun. 2022 Jan 25;13(1):470. doi: 10.1038/s41467-022-28097-y. Nat Commun. 2022. PMID: 35078993 Free PMC article. - Cell cycle arrest determines adult neural stem cell ontogeny by an embryonic Notch-nonoscillatory Hey1 module.
Harada Y, Yamada M, Imayoshi I, Kageyama R, Suzuki Y, Kuniya T, Furutachi S, Kawaguchi D, Gotoh Y. Harada Y, et al. Nat Commun. 2021 Nov 12;12(1):6562. doi: 10.1038/s41467-021-26605-0. Nat Commun. 2021. PMID: 34772946 Free PMC article. - Role of Methylation in Period2 (PER2) Transcription in the Context of the Presence or Absence of Light Signals: Natural and Chemical-Studies on the Pig Model.
Gilun P, Flisikowski K, Flisikowska T, Kwiatkowska J, Wąsowska B, Koziorowska-Gilun M. Gilun P, et al. Int J Mol Sci. 2021 Jul 21;22(15):7796. doi: 10.3390/ijms22157796. Int J Mol Sci. 2021. PMID: 34360562 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases