Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization - PubMed (original) (raw)

Midori Kitazoe, Takashi Maeda, Emiko Nukui, Masakiyo Sakaguchi, Jun Kosaka, Masahiro Miyazaki, Megumi Kosaka, Hiroko Tada, Masaharu Seno, Junzo Sasaki, Nam-Hu Huh, Masayoshi Namba, Hidenori Yamada

Affiliations

PMID: 16233763
DOI: 10.1263/jbb.99.95

Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization

Junichiro Futami et al. J Biosci Bioeng. 2005 Feb.

Abstract

In the post-genomic era, there is pressing need for development of protein manipulation methodology to analyze functions of proteins in living cells. For this purpose, techniques to deliver functional proteins into living cells are currently being evaluated as alternative approaches to the introduction of transcriptionally active DNA. Here, we describe a novel method for efficient protein transduction into living cells in which a protein is simply cationized with polyethylenimine (PEI) by limited chemical conjugation. PEI-cationized proteins appear to adhere to the cell surface by ionic charge interaction and then internalize into cells in a receptor- and transporter-independent fashion. Since PEI is an organic macromolecule with a high cationic-charge density, limited coupling with PEI results in endowment of sufficient cationic charge to proteins without causing serious decline in their fundamental functions. A number of PEI-cationized proteins, such as ribonuclease (RNase), green fluorescent protein (GFP) and immunoglobulin (IgG), efficiently entered cells and functioned in the cytosol. Our results suggest that protein cationization techniques using PEI will be useful for the development of protein transduction technology.

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Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization - PubMed (original) (raw)