Extrasynaptic membrane trafficking regulated by GluR1 serine 845 phosphorylation primes AMPA receptors for long-term potentiation - PubMed (original) (raw)
. 2006 Jan 13;281(2):752-8.
doi: 10.1074/jbc.M509677200. Epub 2005 Nov 4.
Affiliations
- PMID: 16272153
- DOI: 10.1074/jbc.M509677200
Free article
Extrasynaptic membrane trafficking regulated by GluR1 serine 845 phosphorylation primes AMPA receptors for long-term potentiation
Michael C Oh et al. J Biol Chem. 2006.
Free article
Abstract
Enhancement of synaptic transmission, as occurs in long-term potentiation (LTP), can result from several mechanisms that are regulated by phosphorylation of the AMPA-type glutamate receptor (AMPAR). Using a quantitative assay of net serine 845 (Ser-845) phosphorylation in the GluR1 subunit of AMPARs, we investigated the relationship between phospho-Ser-845, GluR1 surface expression, and synaptic strength in hippocampal neurons. About 15% of surface AMPARs in cultured neurons were phosphorylated at Ser-845 basally, whereas chemical potentiation (forskolin/rolipram treatment) persistently increased this to 60% and chemical depression (N-methyl-D-aspartate treatment) decreased it to 10%. These changes in Ser-845 phosphorylation were paralleled by corresponding changes in the surface expression of AMPARs in both cultured neurons and hippocampal slices. For every 1% increase in net phospho-Ser-845, there was 0.75% increase in the surface fraction of GluR1. Phosphorylation of Ser-845 correlated with a selective delivery of AMPARs to extrasynaptic sites, and their synaptic localization required coincident synaptic activity. Furthermore, increasing the extrasynaptic pool of AMPA receptors resulted in stronger theta burst LTP. Our results support a two-step model for delivery of GluR1-containing AMPARs to synapses during activity-dependent LTP, where Ser-845 phosphorylation can traffic AMPARs to extrasynaptic sites for subsequent delivery to synapses during LTP.
Similar articles
- Nobiletin, a citrus flavonoid with neurotrophic action, augments protein kinase A-mediated phosphorylation of the AMPA receptor subunit, GluR1, and the postsynaptic receptor response to glutamate in murine hippocampus.
Matsuzaki K, Miyazaki K, Sakai S, Yawo H, Nakata N, Moriguchi S, Fukunaga K, Yokosuka A, Sashida Y, Mimaki Y, Yamakuni T, Ohizumi Y. Matsuzaki K, et al. Eur J Pharmacol. 2008 Jan 14;578(2-3):194-200. doi: 10.1016/j.ejphar.2007.09.028. Epub 2007 Oct 2. Eur J Pharmacol. 2008. PMID: 17976577 - Input- and subunit-specific AMPA receptor trafficking underlying long-term potentiation at hippocampal CA3 synapses.
Kakegawa W, Tsuzuki K, Yoshida Y, Kameyama K, Ozawa S. Kakegawa W, et al. Eur J Neurosci. 2004 Jul;20(1):101-10. doi: 10.1111/j.1460-9568.2004.03461.x. Eur J Neurosci. 2004. PMID: 15245483 - Benzodiazepine withdrawal-induced glutamatergic plasticity involves up-regulation of GluR1-containing alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptors in Hippocampal CA1 neurons.
Song J, Shen G, Greenfield LJ Jr, Tietz EI. Song J, et al. J Pharmacol Exp Ther. 2007 Aug;322(2):569-81. doi: 10.1124/jpet.107.121798. Epub 2007 May 17. J Pharmacol Exp Ther. 2007. PMID: 17510319 - Regulation of neuronal PKA signaling through AKAP targeting dynamics.
Dell'Acqua ML, Smith KE, Gorski JA, Horne EA, Gibson ES, Gomez LL. Dell'Acqua ML, et al. Eur J Cell Biol. 2006 Jul;85(7):627-33. doi: 10.1016/j.ejcb.2006.01.010. Epub 2006 Feb 28. Eur J Cell Biol. 2006. PMID: 16504338 Review. - Mechanisms by which dopamine receptors may influence synaptic plasticity.
Wolf ME, Mangiavacchi S, Sun X. Wolf ME, et al. Ann N Y Acad Sci. 2003 Nov;1003:241-9. doi: 10.1196/annals.1300.015. Ann N Y Acad Sci. 2003. PMID: 14684450 Review.
Cited by
- A unified computational model for cortical post-synaptic plasticity.
Mäki-Marttunen T, Iannella N, Edwards AG, Einevoll GT, Blackwell KT. Mäki-Marttunen T, et al. Elife. 2020 Jul 30;9:e55714. doi: 10.7554/eLife.55714. Elife. 2020. PMID: 32729828 Free PMC article. - AMPAR trafficking in synapse maturation and plasticity.
Bassani S, Folci A, Zapata J, Passafaro M. Bassani S, et al. Cell Mol Life Sci. 2013 Dec;70(23):4411-30. doi: 10.1007/s00018-013-1309-1. Epub 2013 Mar 9. Cell Mol Life Sci. 2013. PMID: 23475111 Free PMC article. Review. - Long-term depression-associated signaling is required for an in vitro model of NMDA receptor-dependent synapse pruning.
Henson MA, Tucker CJ, Zhao M, Dudek SM. Henson MA, et al. Neurobiol Learn Mem. 2017 Feb;138:39-53. doi: 10.1016/j.nlm.2016.10.013. Epub 2016 Oct 26. Neurobiol Learn Mem. 2017. PMID: 27794462 Free PMC article. - BK channels sustain neuronal Ca2+ oscillations to support hippocampal long-term potentiation and memory formation.
Pham T, Hussein T, Calis D, Bischof H, Skrabak D, Cruz Santos M, Maier S, Spähn D, Kalina D, Simonsig S, Ehinger R, Groschup B, Knipper M, Plesnila N, Ruth P, Lukowski R, Matt L. Pham T, et al. Cell Mol Life Sci. 2023 Nov 21;80(12):369. doi: 10.1007/s00018-023-05016-y. Cell Mol Life Sci. 2023. PMID: 37989805 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases