Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens - PubMed (original) (raw)
Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens
H B van den Hazel et al. Eur J Biochem. 1992.
Free article
Abstract
The Saccharomyces cerevisiae PEP4 gene encodes proteinase A, an aspartyl protease. pep4 mutants are defective in the activation of many vacuolar hydrolases, including proteinase B. We have expressed a pep4 mutation which directs the accumulation of pro-proteinase A with a defective active site. Co-expression with PEP4 leads to normal processing, i.e. the mutant zymogen is functional as a substrate for the maturation reaction in trans. We conclude that wild-type pro-proteinase A has the ability to mediate its own activation. Elimination of the co-expressed PEP4 gene did not effectively stop the processing of the mutant zymogen, owing to a strong, proteinase-B-dependent, phenotypic lag. In a proteinase-B-negative strain, processing of pro-proteinase A led to an active form of a higher molecular mass than the normal mature form.
Similar articles
- The structure and function of Saccharomyces cerevisiae proteinase A.
Parr CL, Keates RA, Bryksa BC, Ogawa M, Yada RY. Parr CL, et al. Yeast. 2007 Jun;24(6):467-80. doi: 10.1002/yea.1485. Yeast. 2007. PMID: 17447722 Review. - Multiple functions of pro-parts of aspartic proteinase zymogens.
Koelsch G, Mares M, Metcalf P, Fusek M. Koelsch G, et al. FEBS Lett. 1994 Apr 18;343(1):6-10. doi: 10.1016/0014-5793(94)80596-2. FEBS Lett. 1994. PMID: 8163018 Review.
Cited by
- Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme.
Christgau S, Kofod LV, Halkier T, Andersen LN, Hockauf M, Dörreich K, Dalbøge H, Kauppinen S. Christgau S, et al. Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):705-12. doi: 10.1042/bj3190705. Biochem J. 1996. PMID: 8920970 Free PMC article. - Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing.
Bauerová V, Hájek M, Pichová I, Hrušková-Heidingsfeldová O. Bauerová V, et al. Folia Microbiol (Praha). 2014 Nov;59(6):485-93. doi: 10.1007/s12223-014-0324-4. Epub 2014 Jun 1. Folia Microbiol (Praha). 2014. PMID: 24880249 - The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani.
Wahleithner JA, Xu F, Brown KM, Brown SH, Golightly EJ, Halkier T, Kauppinen S, Pederson A, Schneider P. Wahleithner JA, et al. Curr Genet. 1996 Mar;29(4):395-403. doi: 10.1007/BF02208621. Curr Genet. 1996. PMID: 8598061 - Metabolic Engineering of Escherichia coli for _para_-Amino-Phenylethanol and _para_-Amino-Phenylacetic Acid Biosynthesis.
Mohammadi Nargesi B, Sprenger GA, Youn JW. Mohammadi Nargesi B, et al. Front Bioeng Biotechnol. 2019 Jan 4;6:201. doi: 10.3389/fbioe.2018.00201. eCollection 2018. Front Bioeng Biotechnol. 2019. PMID: 30662895 Free PMC article. - Mechanism and ion-dependence of in vitro autoactivation of yeast proteinase A: possible implications for compartmentalized activation in vivo.
Van Den Hazel H, Wolff AM, Kielland-Brandt MC, Winther JR. Van Den Hazel H, et al. Biochem J. 1997 Sep 1;326 ( Pt 2)(Pt 2):339-44. Biochem J. 1997. PMID: 9291102 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases