Mutation discovery in bacterial genomes: metronidazole resistance in Helicobacter pylori - PubMed (original) (raw)
Comparative Study
doi: 10.1038/nmeth805. Epub 2005 Nov 18.
Affiliations
- PMID: 16299480
- DOI: 10.1038/nmeth805
Comparative Study
Mutation discovery in bacterial genomes: metronidazole resistance in Helicobacter pylori
Thomas J Albert et al. Nat Methods. 2005 Dec.
Abstract
We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes efficiently, should enrich understanding of systems biology and genome evolution, and help track pathogens during outbreaks.
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