Evidence for a second conserved arginine residue in the integrase family of recombination proteins - PubMed (original) (raw)
Comparative Study
Evidence for a second conserved arginine residue in the integrase family of recombination proteins
K E Abremski et al. Protein Eng. 1992 Jan.
Abstract
This study was designed to search for new regions of similarity in the integrase family of recombination proteins which consists of 28 members found in bacteria and yeast. A computer method based on an information content analysis has been used to align local regions of homology in the set of unaligned protein sequences from this family. Among the aligned regions with high information content were those containing the known conserved histidine, arginine and tyrosine residues. In addition, a new region was identified containing another arginine residue that appears to be conserved in all members of the family. To test further the importance of this newly identified arginine residue, mutants in the Cre protein from phase P1, a member of this integrase family, have been constructed which alter this residue. The mutations which change arginine to lysine and arginine to cysteine depress catalytic activity but not site-specific binding to the lox site. This result is expected for a conserved active site residue. This computer analysis also provides a means for searching for new members of the integrase family.
Similar articles
- A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif.
Matsunami N, Hamaguchi Y, Yamamoto Y, Kuze K, Kangawa K, Matsuo H, Kawaichi M, Honjo T. Matsunami N, et al. Nature. 1989 Dec 21-28;342(6252):934-7. doi: 10.1038/342934a0. Nature. 1989. PMID: 2556644 - Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination.
Liu S, Ma J, Wang W, Zhang M, Xin Q, Peng S, Li R, Zhu H. Liu S, et al. PLoS One. 2010 Jan 25;5(1):e8863. doi: 10.1371/journal.pone.0008863. PLoS One. 2010. PMID: 20111606 Free PMC article. - The integrase family of recombinase: organization and function of the active site.
Grainge I, Jayaram M. Grainge I, et al. Mol Microbiol. 1999 Aug;33(3):449-56. doi: 10.1046/j.1365-2958.1999.01493.x. Mol Microbiol. 1999. PMID: 10577069 Review. - Catalysis by site-specific recombinases.
Stark WM, Boocock MR, Sherratt DJ. Stark WM, et al. Trends Genet. 1992 Dec;8(12):432-9. Trends Genet. 1992. PMID: 1337225 Review.
Cited by
- Insertion sequences.
Mahillon J, Chandler M. Mahillon J, et al. Microbiol Mol Biol Rev. 1998 Sep;62(3):725-74. doi: 10.1128/MMBR.62.3.725-774.1998. Microbiol Mol Biol Rev. 1998. PMID: 9729608 Free PMC article. Review. - Int-B13, an unusual site-specific recombinase of the bacteriophage P4 integrase family, is responsible for chromosomal insertion of the 105-kilobase clc element of Pseudomonas sp. Strain B13.
Ravatn R, Studer S, Zehnder AJ, van der Meer JR. Ravatn R, et al. J Bacteriol. 1998 Nov;180(21):5505-14. doi: 10.1128/JB.180.21.5505-5514.1998. J Bacteriol. 1998. PMID: 9791097 Free PMC article. - Interspecific transfer of mitochondrial genes in fungi and creation of a homologous hybrid gene.
Paquin B, Laforest MJ, Lang BF. Paquin B, et al. Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11807-10. doi: 10.1073/pnas.91.25.11807. Proc Natl Acad Sci U S A. 1994. PMID: 7991539 Free PMC article. - Characterization of the binding sites of two proteins involved in the bacteriophage P2 site-specific recombination system.
Yu A, Haggård-Ljungquist E. Yu A, et al. J Bacteriol. 1993 Mar;175(5):1239-49. doi: 10.1128/jb.175.5.1239-1249.1993. J Bacteriol. 1993. PMID: 8444786 Free PMC article. - Tailed bacteriophages: the order caudovirales.
Ackermann HW. Ackermann HW. Adv Virus Res. 1998;51:135-201. doi: 10.1016/s0065-3527(08)60785-x. Adv Virus Res. 1998. PMID: 9891587 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources