Growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore - PubMed (original) (raw)

Growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore

Colin J Ingham et al. Appl Environ Microbiol. 2005 Dec.

Abstract

A highly porous inorganic material (Anopore) was shown to be an effective support for culturing and imaging a wide range of microorganisms. An inert barrier grid was printed on the rigid surface of Anopore to create a "living chip" of 336 miniaturized compartments (200/cm2) with broad applications in microbial culture.

PubMed Disclaimer

Figures

FIG. 1.

FIG. 1.

Examples of microorganisms grown and imaged on Anopore placed on agar plates. (A) Escherichia coli 2613 stained by Syto-9 and imaged by fluorescence microscopy. (B) Shigella sonnei 2627-1 imaged by scanning electron microscopy, showing the Anopore structure. (C) Candida tropicalis stained with Fun-1 dye. (D) Scanning electron microscopy of Candida albicans showing germ tube outgrowth on Anopore. Bars, 5 μm.

FIG. 2.

FIG. 2.

Growth curve of E. coli 2613 after inoculation on Anopore and incubation on Mueller-Hinton (□) or sheep's blood agar (▪); each time point sample was then stained with Syto-9 and imaged. A total of 160 microcolonies were analyzed per data point; error bars indicate standard deviations from the mean values.

FIG. 3.

FIG. 3.

Detail of 336-compartment Anopore chips. (A) Transmission light microscopy image of uninoculated chip. c, Anopore compartment; g, grid barrier. Bar, 0.8 mm. (B) Fluorescence microscopy image of E. coli 2613 inoculated and cultured in four compartments and subsequently stained with Syto-9.

References

    1. Andersson, H., and A. van den Berg. 2003. Microfluidic devices for cellomics. Sens. Actuators 92**:**315-325.
    1. Binnerup, S. J., O. Højberg, and J. Sørensen. 1998. Gram characteristics determined on single cells and at the microcolony level of bacteria immobilized on polycarbonate membrane filters. J. Microbiol. Methods 31**:**185-192.
    1. Bochner, B. 2003. New technology to assess genotype-phenotype relationships. Nat. Rev. Genet. 4**:**309-314. -PubMed
    1. Bormans, P. 2001. Keramiek. Van baksteen tot synthetisch bot. Amsterdam Natuur Techniek, Amsterdam, The Netherlands.
    1. Bridson, E. Y. 1998. The Oxoid manual, 8th ed. Oxoid Ltd., Basingstoke, Hampshire, United Kingdom.

MeSH terms

LinkOut - more resources