Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder - PubMed (original) (raw)
. 2006 May;290(5):F1143-52.
doi: 10.1152/ajprenal.00333.2005. Epub 2005 Dec 6.
Affiliations
- PMID: 16332929
- DOI: 10.1152/ajprenal.00333.2005
Free article
Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder
Huai Zhen Ruan et al. Am J Physiol Renal Physiol. 2006 May.
Free article
Abstract
The distribution and function of P2X and P2Y receptor subtypes were investigated on intact or cultured intramural ganglia of the cat urinary bladder by immunocytochemistry and calcium-imaging techniques, respectively. Neurons were labeled by all seven P2X receptor subtype antibodies and antibodies for P2Y(2), P2Y(4), P2Y(6), and P2Y(12) receptor subtypes with a staining intensity of immunoreactivity in the following order: P2X(3)=P2Y(2)=P2Y(4)=P2Y(6)=P2Y(12)>P2X(1)=P2X(2)=P2X(4)>P2X(5)=P2X(6)=P2X(7). P2Y(1) receptor antibodies labeled glial cells, but not neurons. P2X(3) and P2Y(4) polyclonal antibodies labeled approximately 95 and 40% of neurons, respectively. Double staining showed that 100, 48.8, and 97.4% of P2X(3) receptor-positive neurons coexpressed choline acetyl transferase (ChAT), nitric oxide synthase (NOS), and neurofilament 200 (NF200), respectively, whereas 100, 59.2, and 97.6% of P2Y(4) receptor-positive neurons coexpressed ChAT, NOS, and NF200, respectively. Application of ATP, alpha,beta-methylene ATP, and uridine triphosphate elevated intracellular Ca(2+) concentration in a subpopulation of dissociated cultured cat intramural ganglia neurons, demonstrating the presence of functional P2Y(4) and P2X(3) receptors. This study indicates that P2X and P2Y receptor subtypes are expressed by cholinergic parasympathetic neurons innervating the urinary bladder. The neurons were also stained for NF200, usually regarded as a marker for large sensory neurons. These novel histochemical properties of cholinergic neurons in the cat bladder suggest that the parasympathetic pathways to the cat bladder may be modulated by complex purinergic synaptic mechanisms.
Similar articles
- Localization of P2X and P2Y receptors in dorsal root ganglia of the cat.
Ruan HZ, Birder LA, de Groat WC, Tai C, Roppolo J, Buffington CA, Burnstock G. Ruan HZ, et al. J Histochem Cytochem. 2005 Oct;53(10):1273-82. doi: 10.1369/jhc.4A6556.2005. Epub 2005 May 27. J Histochem Cytochem. 2005. PMID: 15923368 - Alterations in P2X and P2Y purinergic receptor expression in urinary bladder from normal cats and cats with interstitial cystitis.
Birder LA, Ruan HZ, Chopra B, Xiang Z, Barrick S, Buffington CA, Roppolo JR, Ford AP, de Groat WC, Burnstock G. Birder LA, et al. Am J Physiol Renal Physiol. 2004 Nov;287(5):F1084-91. doi: 10.1152/ajprenal.00118.2004. Epub 2004 Jul 13. Am J Physiol Renal Physiol. 2004. PMID: 15251862 - Increase of intracellular Ca2+ by P2X and P2Y receptor-subtypes in cultured cortical astroglia of the rat.
Fischer W, Appelt K, Grohmann M, Franke H, Nörenberg W, Illes P. Fischer W, et al. Neuroscience. 2009 Jun 2;160(4):767-83. doi: 10.1016/j.neuroscience.2009.02.026. Epub 2009 Mar 13. Neuroscience. 2009. PMID: 19289154 - P2X receptors in health and disease.
Burnstock G, Kennedy C. Burnstock G, et al. Adv Pharmacol. 2011;61:333-72. doi: 10.1016/B978-0-12-385526-8.00011-4. Adv Pharmacol. 2011. PMID: 21586364 Review. - Introduction and perspective, historical note.
Burnstock G. Burnstock G. Front Cell Neurosci. 2013 Nov 21;7:227. doi: 10.3389/fncel.2013.00227. Front Cell Neurosci. 2013. PMID: 24312014 Free PMC article. Review.
Cited by
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.
Fry CH, McCloskey KD. Fry CH, et al. Auton Neurosci. 2021 Nov;235:102852. doi: 10.1016/j.autneu.2021.102852. Epub 2021 Jul 17. Auton Neurosci. 2021. PMID: 34329833 Free PMC article. - Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding.
Gevaert T, Vriens J, Segal A, Everaerts W, Roskams T, Talavera K, Owsianik G, Liedtke W, Daelemans D, Dewachter I, Van Leuven F, Voets T, De Ridder D, Nilius B. Gevaert T, et al. J Clin Invest. 2007 Nov;117(11):3453-62. doi: 10.1172/JCI31766. J Clin Invest. 2007. PMID: 17948126 Free PMC article. - Animal Models for the Investigation of P2X7 Receptors.
Sluyter R, Adriouch S, Fuller SJ, Nicke A, Sophocleous RA, Watson D. Sluyter R, et al. Int J Mol Sci. 2023 May 4;24(9):8225. doi: 10.3390/ijms24098225. Int J Mol Sci. 2023. PMID: 37175933 Free PMC article. Review. - Neural control of the lower urinary tract.
de Groat WC, Griffiths D, Yoshimura N. de Groat WC, et al. Compr Physiol. 2015 Jan;5(1):327-96. doi: 10.1002/cphy.c130056. Compr Physiol. 2015. PMID: 25589273 Free PMC article. Review. - Functions of neuronal P2Y receptors.
Hussl S, Boehm S. Hussl S, et al. Pflugers Arch. 2006 Aug;452(5):538-51. doi: 10.1007/s00424-006-0063-8. Epub 2006 May 10. Pflugers Arch. 2006. PMID: 16691392 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous