Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water - PubMed (original) (raw)
Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water
M Somville. Appl Environ Microbiol. 1984 Dec.
Abstract
The alpha- and beta-glucosidase activity in natural samples can be readily measured during short incubation times (20 min) by using the artificial substrates 4-methylumbelliferyl-alpha-d-glucoside and 4-methylumbelliferyl-beta-d-glucoside. The apparent K(m) of both alpha- and beta-glucosidase for these respective substrates is 0.01 muM. The homologous disaccharides maltose and cellobiose competitively inhibit alpha- and beta-glucosidase, respectively. Absolute substrate specificity of the alpha- and beta-glucosidase is observed with respect to the configuration of carbon atoms 1 and 4. Enrichment cultures on either alpha- and beta-glucoside result in increasing activity of the corresponding glucosidase, both in absolute terms and with respect to the other glucosidase.
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References
- Biochem J. 1956 May;63(1):39-44 - PubMed
- Biochem J. 1955 Dec;61(4):569-74 - PubMed
- Appl Environ Microbiol. 1980 Jun;39(6):1085-95 - PubMed
- J Biol Chem. 1958 Nov;233(5):1113-20 - PubMed
- Biotechnol Bioeng. 1979 Mar;21(3):345-55 - PubMed
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