Effect of growth rate and starvation-survival on the viability and stability of a psychrophilic marine bacterium - PubMed (original) (raw)

Effect of growth rate and starvation-survival on the viability and stability of a psychrophilic marine bacterium

C L Moyer et al. Appl Environ Microbiol. 1989 May.

Abstract

Cell populations of the marine bacterium ANT-300, from either batch or continuous culture with dilution rates ranging from D = 0.015 h to D = 0.200 h, were monitored for viability, direct counts, and optical density for 98 days under starvation conditions. Three stages of starvation survival were observed for each of the cell populations. Although direct counts remained at 2 x 10 to 3 x 10 cells ml throughout the starvation period, large fluctuations occurred in cell viability during stage 1 (0 to 14 days) of starvation survival. Stage 2 (14 to 70 days) involved an overall decrease in viability for each of the cell populations; the rate of viability loss was dependent upon the growth rate. Cell viability stabilized at approximately 0.3% of the direct count in stage 3 (70 to 98 days). Long-term starvation corresponded to the prolongation of stage 3 starvation survival. Cell volumes for each of the cell populations decreased with the length of the starvation period. However, the cell volume of starved cells was also dependent more on growth rate than on the length of the time starved. We hypothesize that the cell population with the slowest growth rate is most closely representative of cells found in the oligotrophic marine environment.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Appl Environ Microbiol. 1977 Apr;33(4):940-6 - PubMed
    1. Microbiol Rev. 1987 Sep;51(3):365-79 - PubMed
    1. J Bacteriol. 1969 Jul;99(1):156-60 - PubMed
    1. Appl Environ Microbiol. 1983 Mar;45(3):1041-8 - PubMed
    1. Appl Environ Microbiol. 1987 Mar;53(3):482-8 - PubMed

LinkOut - more resources