Diverse range of small peptides associated with high-density lipoprotein - PubMed (original) (raw)

Diverse range of small peptides associated with high-density lipoprotein

Glen L Hortin et al. Biochem Biophys Res Commun. 2006.

Abstract

High-density lipoproteins (HDL) were examined as potential carriers of small peptides in plasma. HDL purified by density gradient centrifugation was delipidated and fractionated by size-exclusion chromatography under denaturing conditions. By HPLC and mass spectrometry, more than 100 peptide components were found in the size range from 1000 to 5000 Da. By sequence analysis, peptides were identified as fragments of proteins such as apolipoproteins, fibrinogen, alpha1-proteinase inhibitor, and transthyretin. The results indicate that purified HDL bears a complex range of small peptides. It is unclear whether the peptides have any significant functional role as apolipopeptides, but they may represent a pathway for peptide delivery or scavenging and a significant reservoir of plasma peptides for diagnostic evaluation.

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

Analysis by hplc of 3 pools of HDL-associated peptides. The three pools were from size-exclusion chromatography of HDL-associated peptides with approximate size ranges of 3–6 kDa, panel A; 2–4 kDa, panel B; 1–3 kDa, panel C.

Fig. 2

Fig. 2

Analysis of hplc fractions by MALDI TOF mass spectrometry. Each fraction was collected for 1 min, and 1 μL out of a total of 400 μL for each fraction was analyzed as described in Methods.

Similar articles

Cited by

References

    1. Gotto A, Jr, Pownall HJ, Havel RJ. Introduction to the plasma lipoprteins. Meth Enzymol. 1986;128:3–41. - PubMed
    1. Brewer HB, Jr, Remaley AT, Neufeld EB, Basso F, Joyce C. Regulation of plasma high-density lipoprotein levels by the ABCA1 transporter and the emerging role of high-density lipoprotein in the treatment of cardiovascular disease. Arterioscler Thromb Vasc Biol. 2004;24:1755–1760. - PubMed
    1. Ribaya-Mercado JD, Ordovas JM, Russell RM. Effect of beta-carotene supplementation on the concentrations and distribution of carotenoids, vitamin E, vitamin A, and cholesterol in plasma lipoprotein and non-lipoprotein fractions in healthy older women. J Am Coll Nutr. 1995;14:614–620. - PubMed
    1. Goulinet S, Chapman MJ. Plasma LDL and HDL subspecies are heterogenous in particle content of tocopherols and oxygenated and hydrocarbon carotenoids. Relevance to oxidative resistance and atherogenesis. Arterioscler Thromb Vasc Dis. 1997;17:786–796. - PubMed
    1. Levels JH, Abraham PR, van den Ende A, van Deventer SJ. Distribution and kinetics of lipoprotein-bound endotoxin. Infect Immunol. 2001;69:2821–2828. - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources