Spinal manipulative therapy reduces inflammatory cytokines but not substance P production in normal subjects - PubMed (original) (raw)
Spinal manipulative therapy reduces inflammatory cytokines but not substance P production in normal subjects
Julita A Teodorczyk-Injeyan et al. J Manipulative Physiol Ther. 2006 Jan.
Abstract
Objective: To examine the effect of a single spinal manipulation therapy (SMT) on the in vitro production of inflammatory cytokines, tumor necrosis factor alpha, and interleukin (IL) 1beta, in relation to the systemic (in vivo) levels of neurotransmitter substance P (SP).
Methods: Sixty-four asymptomatic subjects were assigned to SMT, sham manipulation, or venipuncture control group. SMT subjects received a single adjustment in the thoracic spine. Blood and serum samples were obtained from subjects before and then at 20 minutes and 2 hours after intervention. Whole-blood cultures were activated with lipopolysaccharide (LPS) for 24 hours. Cytokine production in culture supernatants and serum SP levels were assessed by specific immunoassays.
Results: Over the study period, a significant proportion (P </= .05) of sham and control subjects demonstrated progressive increases in the synthesis of tumor necrosis factor alpha and IL-1beta. Conversely, in a comparable proportion of cultures from SMT-derived subjects, the production of both cytokines decreased gradually. Normalization of the observed alterations to reflect the changes relative to self-baselines demonstrated that, within 2 hours after intervention, the production of both cytokines increased significantly (P < .001 to .05) in both controls. In contrast, a significant (P < .001 to .05) reduction of proinflammatory cytokine secretion was observed in cultures from SMT-receiving subjects. In all study groups, serum levels of SP remained unaltered within 2 hours after intervention.
Conclusions: SMT-treated subjects show a time-dependent attenuation of LPS-induced production of the inflammatory cytokines unrelated to systemic levels of SP. This suggests SMT-related down-regulation of inflammatory-type responses via a central yet unknown mechanism.
Similar articles
- 17beta-estradiol and progesterone do not influence the production of cytokines from lipopolysaccharide-stimulated monocytes in humans.
Bouman A, Schipper M, Heineman MJ, Faas M. Bouman A, et al. Fertil Steril. 2004 Oct;82 Suppl 3:1212-9. doi: 10.1016/j.fertnstert.2004.05.072. Fertil Steril. 2004. PMID: 15474098 - The effects of CO2 on cytokine concentrations in endotoxin-stimulated human whole blood.
Kimura D, Totapally BR, Raszynski A, Ramachandran C, Torbati D. Kimura D, et al. Crit Care Med. 2008 Oct;36(10):2823-7. doi: 10.1097/CCM.0b013e318186f556. Crit Care Med. 2008. PMID: 18766096 Clinical Trial. - Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE. Shahbakhti H, et al. Photochem Photobiol. 2004 Sep-Oct;80(2):231-5. doi: 10.1562/2004-01-27-RA-066. Photochem Photobiol. 2004. PMID: 15362934 Clinical Trial. - Concentration-dependent roles for heparin in modifying lipopolysaccharide-induced activation of mononuclear cells in whole blood.
Hochart H, Jenkins PV, Preston RJ, Smith OP, White B, O'Donnell J. Hochart H, et al. Thromb Haemost. 2008 Mar;99(3):570-5. doi: 10.1160/TH07-06-0424. Thromb Haemost. 2008. PMID: 18327406 - Association between serum values of C-reactive protein and cytokine production in whole blood of patients with type 2 diabetes.
Castoldi G, Galimberti S, Riva C, Papagna R, Querci F, Casati M, Zerbini G, Caccianiga G, Ferrarese C, Baldoni M, Valsecchi MG, Stella A. Castoldi G, et al. Clin Sci (Lond). 2007 Jul;113(2):103-8. doi: 10.1042/CS20060338. Clin Sci (Lond). 2007. PMID: 17362204
Cited by
- Quantification of cavitation and gapping of lumbar zygapophyseal joints during spinal manipulative therapy.
Cramer GD, Ross K, Raju PK, Cambron J, Cantu JA, Bora P, Dexheimer JM, McKinnis R, Habeck AR, Selby S, Pocius JD, Gregerson D. Cramer GD, et al. J Manipulative Physiol Ther. 2012 Oct;35(8):614-21. doi: 10.1016/j.jmpt.2012.06.007. Epub 2012 Aug 14. J Manipulative Physiol Ther. 2012. PMID: 22902194 Free PMC article. Clinical Trial. - Effects of spinal manipulative therapy on inflammatory mediators in patients with non-specific low back pain: a non-randomized controlled clinical trial.
Teodorczyk-Injeyan JA, Triano JJ, Gringmuth R, DeGraauw C, Chow A, Injeyan HS. Teodorczyk-Injeyan JA, et al. Chiropr Man Therap. 2021 Jan 8;29(1):3. doi: 10.1186/s12998-020-00357-y. Chiropr Man Therap. 2021. PMID: 33413508 Free PMC article. Clinical Trial. - Knowledge, beliefs, and attitudes of spinal manipulation: a cross-sectional survey of Italian physiotherapists.
Mourad F, Yousif MS, Maselli F, Pellicciari L, Meroni R, Dunning J, Puentedura E, Taylor A, Kerry R, Hutting N, Kranenburg HA. Mourad F, et al. Chiropr Man Therap. 2022 Sep 12;30(1):38. doi: 10.1186/s12998-022-00449-x. Chiropr Man Therap. 2022. PMID: 36096835 Free PMC article. - Short-term response of hip mobilizations and exercise in individuals with chronic low back pain: a case series.
Burns SA, Mintken PE, Austin GP, Cleland J. Burns SA, et al. J Man Manip Ther. 2011 May;19(2):100-7. doi: 10.1179/2042618610Y.0000000007. J Man Manip Ther. 2011. PMID: 22547920 Free PMC article. - Time to evolve: the applicability of pain phenotyping in manual therapy.
Damian K, Chad C, Kenneth L, David G. Damian K, et al. J Man Manip Ther. 2022 Apr;30(2):61-67. doi: 10.1080/10669817.2022.2052560. J Man Manip Ther. 2022. PMID: 35344468 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical