A PIF-dependent recombinogenic signal in the mitochondrial DNA of yeast - PubMed (original) (raw)
A PIF-dependent recombinogenic signal in the mitochondrial DNA of yeast
F Foury et al. EMBO J. 1985.
Abstract
From their recombination properties, tandem rho mutants of the mitochondrial genome of Saccharomyces cerevisiae were divided into two categories. In crosses between PIF-independent rho and rho strains, the recombination frequency is low and similar in PIF/pif and pif/pif diploids. In crosses between PIF-dependent rho and rho strains, the recombination frequency is stimulated 10-50 times in PIF/pif diploids and is drastically decreased in pif/pif diploids. These results suggest that a recombinogenic signal is present in the mitochondrial (mt) DNA of PIF-dependent rho clones. This signal is not recognized in pif mutants. Sequence analysis of a series of small (<300 bp) overlapping tandem rho genomes located in the ery region of the 21S rRNA gene led us to identify an essential element of this signal within a 41-bp A+T sequence exhibiting over 26 bp a perfect dyad symmetry. However the recombinogenic signal is not sequence-specific since the sequence described above does not characterize PIF-dependent rho clones located in the oli1 region. Our results rather suggest that the recombinogenic signal is related to the topology of rho DNA. Denaturated sites in the double helix or cruciform structures elicited by local negative supercoiling might be preferred sites of the initiation of recombination.
Similar articles
- pif mutation blocks recombination between mitochondrial rho+ and rho- genomes having tandemly arrayed repeat units in Saccharomyces cerevisiae.
Foury F, Kolodynski J. Foury F, et al. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5345-9. doi: 10.1073/pnas.80.17.5345. Proc Natl Acad Sci U S A. 1983. PMID: 6310571 Free PMC article. - Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA.
Foury F, Lahaye A. Foury F, et al. EMBO J. 1987 May;6(5):1441-9. doi: 10.1002/j.1460-2075.1987.tb02385.x. EMBO J. 1987. PMID: 3038524 Free PMC article. - In vivo double-strand breaks occur at recombinogenic G + C-rich sequences in the yeast mitochondrial genome.
Zinn AR, Pohlman JK, Perlman PS, Butow RA. Zinn AR, et al. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2686-90. doi: 10.1073/pnas.85.8.2686. Proc Natl Acad Sci U S A. 1988. PMID: 3282235 Free PMC article. - Lack of site-specific recombination between mitochondrial genomes of petite mutants of yeast.
Rayko E, Goursot R, Bernardi G. Rayko E, et al. Gene. 1993 Oct 15;132(2):167-74. doi: 10.1016/0378-1119(93)90192-6. Gene. 1993. PMID: 8224860
Cited by
- A DNA helicase required for maintenance of the functional mitochondrial genome in Saccharomyces cerevisiae.
Sedman T, Kuusk S, Kivi S, Sedman J. Sedman T, et al. Mol Cell Biol. 2000 Mar;20(5):1816-24. doi: 10.1128/MCB.20.5.1816-1824.2000. Mol Cell Biol. 2000. PMID: 10669756 Free PMC article. - Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA.
Ramanagoudr-Bhojappa R, Chib S, Byrd AK, Aarattuthodiyil S, Pandey M, Patel SS, Raney KD. Ramanagoudr-Bhojappa R, et al. J Biol Chem. 2013 May 31;288(22):16185-95. doi: 10.1074/jbc.M113.470013. Epub 2013 Apr 17. J Biol Chem. 2013. PMID: 23596008 Free PMC article. - Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain.
Gu Y, Masuda Y, Kamiya K. Gu Y, et al. Nucleic Acids Res. 2008 Nov;36(19):6295-308. doi: 10.1093/nar/gkn609. Epub 2008 Oct 3. Nucleic Acids Res. 2008. PMID: 18835853 Free PMC article. - An enzyme in yeast mitochondria that catalyzes a step in branched-chain amino acid biosynthesis also functions in mitochondrial DNA stability.
Zelenaya-Troitskaya O, Perlman PS, Butow RA. Zelenaya-Troitskaya O, et al. EMBO J. 1995 Jul 3;14(13):3268-76. doi: 10.1002/j.1460-2075.1995.tb07330.x. EMBO J. 1995. PMID: 7621838 Free PMC article. - Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase.
Aarattuthodiyil S, Byrd AK, Raney KD. Aarattuthodiyil S, et al. Nucleic Acids Res. 2014 Oct;42(18):11707-20. doi: 10.1093/nar/gku845. Epub 2014 Sep 23. Nucleic Acids Res. 2014. PMID: 25249618 Free PMC article.
References
- Nature. 1978 Dec 7;276(5688):577-83 - PubMed
- Nature. 1981 Feb 5;289(5797):466-70 - PubMed
- FEBS Lett. 1979 Dec 15;108(2):429-32 - PubMed
- Biochimie. 1979;61(9):985-1010 - PubMed
- Genetics. 1976 Apr;82(4):645-63 - PubMed
LinkOut - more resources
Full Text Sources
Molecular Biology Databases