Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor - PubMed (original) (raw)

Comparative Study

Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor

R G Goodwin et al. Mol Cell Biol. 1991 Jun.

Abstract

Clones encoding the type 1 (p80) and type 2 (p60) forms of the murine receptors for tumor necrosis factor (TNF) were isolated by cross-hybridization using probes derived from the cloned human TNF receptors. Each of the murine receptors shows strong sequence homology to the corresponding human receptor (approximately 65% amino acid identity) throughout the molecule but only modest homology, limited to ligand-binding domains, between themselves. The ligand-binding characteristics of the recombinant murine receptors mirror those of the human homologs: both receptor types bind TNF-alpha and -beta with multiple affinity classes, and the ligands cross-compete. Analysis of the murine transcripts encoding these receptors revealed the presence of RNAs for one or both forms of the receptors in all cells examined. It was also demonstrated that for both types of human TNF receptor, variably sized transcripts are observed in different cells. The murine cDNAs were further used to determine the chromosomal locations of the TNF receptor genes. They are not linked, in contrast to the ligands, and map to chromosomes 4 (type 1) and 6 (type 2).

PubMed Disclaimer

Similar articles

• Soluble forms of tumor necrosis factor receptors (TNF-Rs). The cDNA for the type I TNF-R, cloned using amino acid sequence data of its soluble form, encodes both the cell surface and a soluble form of the receptor.
  Nophar Y, Kemper O, Brakebusch C, Englemann H, Zwang R, Aderka D, Holtmann H, Wallach D. Nophar Y, et al. EMBO J. 1990 Oct;9(10):3269-78. doi: 10.1002/j.1460-2075.1990.tb07526.x. EMBO J. 1990. PMID: 1698610 Free PMC article.
• Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific.
  Lewis M, Tartaglia LA, Lee A, Bennett GL, Rice GC, Wong GH, Chen EY, Goeddel DV. Lewis M, et al. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2830-4. doi: 10.1073/pnas.88.7.2830. Proc Natl Acad Sci U S A. 1991. PMID: 1849278 Free PMC article.
• Molecular cloning and expression of a receptor for human tumor necrosis factor.
  Schall TJ, Lewis M, Koller KJ, Lee A, Rice GC, Wong GH, Gatanaga T, Granger GA, Lentz R, Raab H, et al. Schall TJ, et al. Cell. 1990 Apr 20;61(2):361-70. doi: 10.1016/0092-8674(90)90816-w. Cell. 1990. PMID: 2158863
• Tumor necrosis factor ligand superfamily: involvement in the pathology of malignant lymphomas.
  Gruss HJ, Dower SK. Gruss HJ, et al. Blood. 1995 Jun 15;85(12):3378-404. Blood. 1995. PMID: 7780126 Review.
• Two TNF receptors.
  Tartaglia LA, Goeddel DV. Tartaglia LA, et al. Immunol Today. 1992 May;13(5):151-3. doi: 10.1016/0167-5699(92)90116-O. Immunol Today. 1992. PMID: 1322675 Review.

Cited by

• Comparative map for mice and humans.
  Nadeau JH, Davisson MT, Doolittle DP, Grant P, Hillyard AL, Kosowsky MR, Roderick TH. Nadeau JH, et al. Mamm Genome. 1992;3(9):480-536. doi: 10.1007/BF00778825. Mamm Genome. 1992. PMID: 1392257 Review. No abstract available.
• A deletion polymorphism in the human alpha-2-macroglobulin (A2M) gene.
  Matthijs G, Marynen P. Matthijs G, et al. Nucleic Acids Res. 1991 Sep 25;19(18):5102. doi: 10.1093/nar/19.18.5102. Nucleic Acids Res. 1991. PMID: 1717945 Free PMC article.
• Etk/Bmx as a tumor necrosis factor receptor type 2-specific kinase: role in endothelial cell migration and angiogenesis.
  Pan S, An P, Zhang R, He X, Yin G, Min W. Pan S, et al. Mol Cell Biol. 2002 Nov;22(21):7512-23. doi: 10.1128/MCB.22.21.7512-7523.2002. Mol Cell Biol. 2002. PMID: 12370298 Free PMC article.
• Expression and localization of messenger RNA for tumor necrosis factor receptor (TNF-R) I and TNF-RII in pregnant mouse uterus and placenta.
  Roby KF, Laham N, Kröning H, Terranova PF, Hunt JS. Roby KF, et al. Endocrine. 1995 Aug;3(8):557-62. doi: 10.1007/BF02953019. Endocrine. 1995. PMID: 21153132
• Mouse chromosome 6.
  Elliott RW, Moore KJ. Elliott RW, et al. Mamm Genome. 1992;3 Spec No:S81-103. doi: 10.1007/BF00648424. Mamm Genome. 1992. PMID: 1498445 Review. No abstract available.

References

1. 1. Nucleic Acids Res. 1989 Jul 25;17(14):5489-500 - PubMed
2. 1. J Immunol. 1985 Sep;135(3):2069-73 - PubMed
3. 1. Oncogene Res. 1988;2(2):149-65 - PubMed
4. 1. Cytogenet Cell Genet. 1986;43(3-4):181-6 - PubMed
5. 1. Eur J Biochem. 1986 Nov 17;161(1):177-84 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)