Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene - PubMed (original) (raw)
Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
S D Lupton et al. Mol Cell Biol. 1991 Jun.
Abstract
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
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References
- Hum Gene Ther. 1990 Summer;1(2):125-34 - PubMed
- Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 - PubMed
- Antimicrob Agents Chemother. 1987 Jun;31(6):844-9 - PubMed
- Mol Cell Biol. 1988 Apr;8(4):1803-8 - PubMed
- J Virol. 1985 Nov;56(2):512-9 - PubMed
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