Surface-induced changes in protein adsorption and implications for cellular phenotypic responses to surface interaction - PubMed (original) (raw)
Surface-induced changes in protein adsorption and implications for cellular phenotypic responses to surface interaction
Lorcan T Allen et al. Biomaterials. 2006 Jun.
Abstract
Understanding external factors that determine cellular phenotypic responses is of key interest in the field of biomaterials. Currently, material surface characteristics, protein adsorption and cellular phenotypic responses are all considered to be interrelated and ultimately determine the biocompatibility of materials. The exact nature of the relationship between these distinct, yet related, phenomena still remains to be elucidated. Through the use of a series of thermoresponsive N-isopropylacrylamide-based co-polymer films, we aimed to shed light on the relationship between surface hydrophobicity, protein adsorption and subsequent cellular response. Despite changes in co-polymer hydrophobicity mediated by altered ratios of constituent monomers, differential cellular response was only apparent in the presence of serum. Co-polymer films displayed alterations with respect to the amount of protein adsorbed on the surface, with individual serum proteins (albumin and fibronectin) displaying contrasting adsorption characteristics. Changes in protein adsorption corresponded to changes in cell adhesion, cytoskeletal organisation and cell morphology, as well as to changes in cell movement and intracellular signalling events. Examination of focal adhesion kinase (FAK), and extracellular signal-regulated kinase (ERK 1/2), important mediators of adhesion and growth factor-related signalling events, revealed a comparative reduction in phosphorylation of these signalling proteins in cells grown on co-polymers in comparison to those cultured on tissue culture polystyrene (TCP; used as a control surface). We also associated surface-mediated phenotypic alterations of cells grown on TCP and co-polymer films with particular changes in gene expression. These results indicate that cellular response to interaction with our series of co-polymer films is determined by the surface-adsorbed protein layer, which in turn is determined by the changing surface chemistry as the ratio of the co-monomers is altered.
Similar articles
- Analysis of the biological response of endothelial and fibroblast cells cultured on synthetic scaffolds with various hydrophilic/hydrophobic ratios: influence of fibronectin adsorption and conformation.
Campillo-Fernández AJ, Unger RE, Peters K, Halstenberg S, Santos M, Salmerón Sánchez M, Meseguer Dueñas JM, Monleón Pradas M, Gómez Ribelles JL, Kirkpatrick CJ. Campillo-Fernández AJ, et al. Tissue Eng Part A. 2009 Jun;15(6):1331-41. doi: 10.1089/ten.tea.2008.0146. Tissue Eng Part A. 2009. PMID: 18976156 - The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers.
Barrias CC, Martins MC, Almeida-Porada G, Barbosa MA, Granja PL. Barrias CC, et al. Biomaterials. 2009 Jan;30(3):307-16. doi: 10.1016/j.biomaterials.2008.09.048. Epub 2008 Oct 25. Biomaterials. 2009. PMID: 18952279 - Adhesion, actin cytoskeleton organisation and the spreading of colon adenocarcinoma cells induced by EGF are mediated by alpha2beta1 integrin low clustering through focal adhesion kinase.
Pichard V, Honoré S, Kovacic H, Li C, Prevôt C, Briand C, Rognoni JB. Pichard V, et al. Histochem Cell Biol. 2001 Oct;116(4):337-48. doi: 10.1007/s004180100324. Histochem Cell Biol. 2001. PMID: 11702192 - Mediation of biomaterial-cell interactions by adsorbed proteins: a review.
Wilson CJ, Clegg RE, Leavesley DI, Pearcy MJ. Wilson CJ, et al. Tissue Eng. 2005 Jan-Feb;11(1-2):1-18. doi: 10.1089/ten.2005.11.1. Tissue Eng. 2005. PMID: 15738657 Review. - Biomimetic approaches to modulate cellular adhesion in biomaterials: A review.
Rahmany MB, Van Dyke M. Rahmany MB, et al. Acta Biomater. 2013 Mar;9(3):5431-7. doi: 10.1016/j.actbio.2012.11.019. Epub 2012 Nov 21. Acta Biomater. 2013. PMID: 23178862 Review.
Cited by
- Graphene-Related Nanomaterials for Biomedical Applications.
Lazăr AI, Aghasoleimani K, Semertsidou A, Vyas J, Roșca AL, Ficai D, Ficai A. Lazăr AI, et al. Nanomaterials (Basel). 2023 Mar 17;13(6):1092. doi: 10.3390/nano13061092. Nanomaterials (Basel). 2023. PMID: 36985986 Free PMC article. Review. - Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models.
Busch M, Brouwer H, Aalderink G, Bredeck G, Kämpfer AAM, Schins RPF, Bouwmeester H. Busch M, et al. Front Toxicol. 2023 Jan 27;5:1112212. doi: 10.3389/ftox.2023.1112212. eCollection 2023. Front Toxicol. 2023. PMID: 36777263 Free PMC article. Review. - A Biodegradable Polymeric Matrix for the Repair of Annulus Fibrosus Defects in Intervertebral Discs.
Saghari Fard MR, Krueger JP, Stich S, Berger P, Kühl AA, Sittinger M, Hartwig T, Endres M. Saghari Fard MR, et al. Tissue Eng Regen Med. 2022 Dec;19(6):1311-1320. doi: 10.1007/s13770-022-00466-0. Epub 2022 Jul 11. Tissue Eng Regen Med. 2022. PMID: 35816226 Free PMC article. - Static and Dynamic Biomaterial Engineering for Cell Modulation.
Park HJ, Hong H, Thangam R, Song MG, Kim JE, Jo EH, Jang YJ, Choi WH, Lee MY, Kang H, Lee KB. Park HJ, et al. Nanomaterials (Basel). 2022 Apr 17;12(8):1377. doi: 10.3390/nano12081377. Nanomaterials (Basel). 2022. PMID: 35458085 Free PMC article. Review. - Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium.
Zhong J, Li X, Yao Y, Zhou J, Cao S, Zhang X, Jian Y, Zhao K. Zhong J, et al. J Mater Sci Mater Med. 2022 Feb 2;33(2):20. doi: 10.1007/s10856-022-06646-7. J Mater Sci Mater Med. 2022. PMID: 35107647 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous