Specific monocyte adhesion to endothelial cells induced by oxidized phospholipids involves activation of cPLA2 and lipoxygenase - PubMed (original) (raw)
. 2006 May;47(5):1054-62.
doi: 10.1194/jlr.M500555-JLR200. Epub 2006 Feb 5.
Affiliations
- PMID: 16461778
- DOI: 10.1194/jlr.M500555-JLR200
Free article
Specific monocyte adhesion to endothelial cells induced by oxidized phospholipids involves activation of cPLA2 and lipoxygenase
Joakim Huber et al. J Lipid Res. 2006 May.
Free article
Abstract
Oxidized phospholipids stimulate endothelial cells to bind monocytes, but not neutrophils, an initiating event in atherogenesis. Here, we investigate intracellular signaling events induced by oxidized phospholipids in human umbilical vein endothelial cells (HUVECs) that lead to specific monocyte adhesion. In a static adhesion assay, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine and one of its components, 1-palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine, stimulated HUVECs to bind U937 cells and human peripheral blood monocytes but not HL-60 cells or blood neutrophils. Monocyte adhesion was dependent on protein kinases A and C, extracellular signal-regulated kinase 1/2, p38 mitogen activated protein kinases (MAPKs), and cytosolic phospholipase A(2) (cPLA(2)). Inhibition of 12-lipoxygenase (12-LOX), but not cyclooxygenases, blocked monocyte adhesion, and addition of 12-hydroxyeicosatetraenoic acid (12-HETE) mimicked the effects of oxidized phospholipids. Peroxisome proliferator-activated receptor alpha (PPARalpha) was excluded as a possible target for 12-HETE, because monocyte adhesion was still induced in endothelial cells from PPARalpha null mice. Together, our results suggest that oxidized phospholipids stimulate HUVECs to specifically bind monocytes involving MAPK pathways, which lead to the activation of cPLA(2) and 12-LOX. Further analysis of signaling pathways induced by oxidized phospholipids that lead to specific monocyte adhesion should ultimately lead to the development of novel therapeutic approaches against chronic inflammatory diseases.
Similar articles
- Structurally similar oxidized phospholipids differentially regulate endothelial binding of monocytes and neutrophils.
Leitinger N, Tyner TR, Oslund L, Rizza C, Subbanagounder G, Lee H, Shih PT, Mackman N, Tigyi G, Territo MC, Berliner JA, Vora DK. Leitinger N, et al. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):12010-5. doi: 10.1073/pnas.96.21.12010. Proc Natl Acad Sci U S A. 1999. PMID: 10518567 Free PMC article. - Oxidized cholesteryl linoleates stimulate endothelial cells to bind monocytes via the extracellular signal-regulated kinase 1/2 pathway.
Huber J, Boechzelt H, Karten B, Surboeck M, Bochkov VN, Binder BR, Sattler W, Leitinger N. Huber J, et al. Arterioscler Thromb Vasc Biol. 2002 Apr 1;22(4):581-6. doi: 10.1161/01.atv.0000012782.59850.41. Arterioscler Thromb Vasc Biol. 2002. PMID: 11950694 - 15S-Lipoxygenase-2 mediates arachidonic acid-stimulated adhesion of human breast carcinoma cells through the activation of TAK1, MKK6, and p38 MAPK.
Nony PA, Kennett SB, Glasgow WC, Olden K, Roberts JD. Nony PA, et al. J Biol Chem. 2005 Sep 9;280(36):31413-9. doi: 10.1074/jbc.M500418200. Epub 2005 Jul 6. J Biol Chem. 2005. PMID: 16000313 - Postprandial lipoproteins and the molecular regulation of vascular homeostasis.
Botham KM, Wheeler-Jones CP. Botham KM, et al. Prog Lipid Res. 2013 Oct;52(4):446-64. doi: 10.1016/j.plipres.2013.06.001. Epub 2013 Jun 15. Prog Lipid Res. 2013. PMID: 23774609 Review. - Physiological effects of oxidized phospholipids and their cellular signaling mechanisms in inflammation.
Greig FH, Kennedy S, Spickett CM. Greig FH, et al. Free Radic Biol Med. 2012 Jan 15;52(2):266-80. doi: 10.1016/j.freeradbiomed.2011.10.481. Epub 2011 Oct 31. Free Radic Biol Med. 2012. PMID: 22080084 Review.
Cited by
- Therapeutic potential of paeoniflorin in atherosclerosis: A cellular action and mechanism-based perspective.
Yu W, Ilyas I, Hu X, Xu S, Yu H. Yu W, et al. Front Immunol. 2022 Dec 21;13:1072007. doi: 10.3389/fimmu.2022.1072007. eCollection 2022. Front Immunol. 2022. PMID: 36618414 Free PMC article. Review. - A review on therapeutical potential of paeonol in atherosclerosis.
Yu W, Ilyas I, Aktar N, Xu S. Yu W, et al. Front Pharmacol. 2022 Aug 4;13:950337. doi: 10.3389/fphar.2022.950337. eCollection 2022. Front Pharmacol. 2022. PMID: 35991897 Free PMC article. Review. - Oxidized Phospholipids in Healthy and Diseased Lung Endothelium.
Karki P, Birukov KG. Karki P, et al. Cells. 2020 Apr 15;9(4):981. doi: 10.3390/cells9040981. Cells. 2020. PMID: 32326516 Free PMC article. Review. - Redox (phospho)lipidomics of signaling in inflammation and programmed cell death.
Tyurina YY, St Croix CM, Watkins SC, Watson AM, Epperly MW, Anthonymuthu TS, Kisin ER, Vlasova II, Krysko O, Krysko DV, Kapralov AA, Dar HH, Tyurin VA, Amoscato AA, Popova EN, Bolevich SB, Timashev PS, Kellum JA, Wenzel SE, Mallampalli RK, Greenberger JS, Bayir H, Shvedova AA, Kagan VE. Tyurina YY, et al. J Leukoc Biol. 2019 Jul;106(1):57-81. doi: 10.1002/JLB.3MIR0119-004RR. Epub 2019 May 9. J Leukoc Biol. 2019. PMID: 31071242 Free PMC article. Review. - Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells.
Kukumberg M, Yao Y, Goh SH, Neo DJ, Yao JY, Yim EK. Kukumberg M, et al. Adv Biosyst. 2018 Jun;2(6):1700217. doi: 10.1002/adbi.201700217. Epub 2018 Apr 24. Adv Biosyst. 2018. PMID: 30766915 Free PMC article.