The NuRD component Mbd3 is required for pluripotency of embryonic stem cells - PubMed (original) (raw)

doi: 10.1038/ncb1372. Epub 2006 Feb 5.

Affiliations

PMID: 16462733
DOI: 10.1038/ncb1372

The NuRD component Mbd3 is required for pluripotency of embryonic stem cells

Keisuke Kaji et al. Nat Cell Biol. 2006 Mar.

Abstract

Cells of early mammalian embryos have the potential to develop into any adult cell type, and are thus said to be pluripotent. Pluripotency is lost during embryogenesis as cells commit to specific developmental pathways. Although restriction of developmental potential is often associated with repression of inappropriate genetic programmes, the role of epigenetic silencing during early lineage commitment remains undefined. Here, we used mouse embryonic stem cells to study the function of epigenetic silencing in pluripotent cells. Embryonic stem cells lacking Mbd3 - a component of the nucleosome remodelling and histone deacetylation (NuRD) complex - were viable but failed to completely silence genes that are expressed before implantation of the embryo. Mbd3-deficient embryonic stem cells could be maintained in the absence of leukaemia inhibitory factor (LIF) and could initiate differentiation in embryoid bodies or chimeric embryos, but failed to commit to developmental lineages. Our findings define a role for epigenetic silencing in the cell-fate commitment of pluripotent cells.

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Repressed by a NuRD.
Crook JM, Dunn NR, Colman A. Crook JM, et al. Nat Cell Biol. 2006 Mar;8(3):212-4. doi: 10.1038/ncb0306-212. Nat Cell Biol. 2006. PMID: 16508673 No abstract available.

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The NuRD component Mbd3 is required for pluripotency of embryonic stem cells - PubMed (original) (raw)