High level transient expression of the murine coronavirus haemagglutinin-esterase - PubMed (original) (raw)
High level transient expression of the murine coronavirus haemagglutinin-esterase
M Pfleiderer et al. J Gen Virol. 1991 Jun.
Abstract
We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.
Similar articles
- Functional analysis of the coronavirus MHV-JHM surface glycoproteins in vaccinia virus recombinants.
Pfleiderer M, Routledge E, Siddell SG. Pfleiderer M, et al. Adv Exp Med Biol. 1990;276:21-31. doi: 10.1007/978-1-4684-5823-7_4. Adv Exp Med Biol. 1990. PMID: 2103098 No abstract available. - The haemagglutinin of bovine coronavirus exhibits significant similarity to the haemagglutinin of type C influenza virus.
Parker MD, Cox GJ, Yoo DW, Fitzpatrick DR, Babiuk LA. Parker MD, et al. Adv Exp Med Biol. 1990;276:103-8. doi: 10.1007/978-1-4684-5823-7_15. Adv Exp Med Biol. 1990. PMID: 2103095 No abstract available. - Heterogeneity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses.
Yokomori K, Banner LR, Lai MM. Yokomori K, et al. Virology. 1991 Aug;183(2):647-57. doi: 10.1016/0042-6822(91)90994-m. Virology. 1991. PMID: 1649505 Free PMC article. - Structure and function of the HEF glycoprotein of influenza C virus.
Herrler G, Klenk HD. Herrler G, et al. Adv Virus Res. 1991;40:213-34. doi: 10.1016/s0065-3527(08)60280-8. Adv Virus Res. 1991. PMID: 1957719 Free PMC article. Review. - Background paper. Coronavirus M and HE: two peculiar glycoproteins.
Rottier PJ. Rottier PJ. Adv Exp Med Biol. 1990;276:91-4. doi: 10.1007/978-1-4684-5823-7_13. Adv Exp Med Biol. 1990. PMID: 2103107 Review. No abstract available.
Cited by
- Attachment of mouse hepatitis virus to O-acetylated sialic acid is mediated by hemagglutinin-esterase and not by the spike protein.
Langereis MA, van Vliet AL, Boot W, de Groot RJ. Langereis MA, et al. J Virol. 2010 Sep;84(17):8970-4. doi: 10.1128/JVI.00566-10. Epub 2010 Jun 10. J Virol. 2010. PMID: 20538854 Free PMC article. - Structure, function and evolution of the hemagglutinin-esterase proteins of corona- and toroviruses.
de Groot RJ. de Groot RJ. Glycoconj J. 2006 Feb;23(1-2):59-72. doi: 10.1007/s10719-006-5438-8. Glycoconj J. 2006. PMID: 16575523 Free PMC article. Review. - Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus.
Weiss SR, Navas-Martin S. Weiss SR, et al. Microbiol Mol Biol Rev. 2005 Dec;69(4):635-64. doi: 10.1128/MMBR.69.4.635-664.2005. Microbiol Mol Biol Rev. 2005. PMID: 16339739 Free PMC article. Review. - Expression of hemagglutinin esterase protein from recombinant mouse hepatitis virus enhances neurovirulence.
Kazi L, Lissenberg A, Watson R, de Groot RJ, Weiss SR. Kazi L, et al. J Virol. 2005 Dec;79(24):15064-73. doi: 10.1128/JVI.79.24.15064-15073.2005. J Virol. 2005. PMID: 16306577 Free PMC article. - Luxury at a cost? Recombinant mouse hepatitis viruses expressing the accessory hemagglutinin esterase protein display reduced fitness in vitro.
Lissenberg A, Vrolijk MM, van Vliet AL, Langereis MA, de Groot-Mijnes JD, Rottier PJ, de Groot RJ. Lissenberg A, et al. J Virol. 2005 Dec;79(24):15054-63. doi: 10.1128/JVI.79.24.15054-15063.2005. J Virol. 2005. PMID: 16306576 Free PMC article.