Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens - PubMed (original) (raw)

. 1991 Jun;138(6):1461-9.

Affiliations

• PMID: 1647139
• PMCID: PMC1886387

Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens

T C Wu et al. Am J Pathol. 1991 Jun.

Abstract

The highly restricted expression of the Epstein-Barr virus (EBV) genome in malignancy has limited the use of EBV detection methods applicable to formalin-fixed paraffin-embedded carcinoma specimens. In EBV-transformed lymphocytes very short nonprotein coding EBV transcripts (EBERs) are expressed in much higher abundance (10(7) copies per cell) than other EBV latency transcripts. Using a 3H riboprobe, the authors demonstrated EBER1 expression in NASOPHARYNGEAL CARCINOMAS (NPCs) as well as in parotid salivary gland. Recognition of EBER1 expression was facilitated by the intensity of hybridization and its characteristic morphology (nuclear with nucleolar sparing). EBER1 expression was not demonstrated in other epithelial malignancies arising from mucosal surfaces (oropharynx, uterine cervix) from which EBV shedding has been detected. Repeat study of the NPC specimens with digoxigenin-labeled probe yielded hybridization signal with subcellular morphologic detail and without background in a 12-hour procedure. Thus the EBER1 transcript is an appropriate target for in situ hybridization detection of EBV in formalin-fixed paraffin-embedded carcinoma specimens.

PubMed Disclaimer

Similar articles

• Expression of EBER1 in primary and metastatic nasopharyngeal carcinoma tissues using in situ hybridization. A correlation with WHO histologic subtypes.
  Tsai ST, Jin YT, Su IJ. Tsai ST, et al. Cancer. 1996 Jan 15;77(2):231-6. doi: 10.1002/(SICI)1097-0142(19960115)77:2<231::AID-CNCR2>3.0.CO;2-P. Cancer. 1996. PMID: 8625228
• Expression of Epstein-Barr virus-encoded small nuclear RNA 1 in Japanese nasopharyngeal carcinomas.
  Inoue H, Sato Y, Tsuchiya B, Nagai H, Takahashi H, Kameya T. Inoue H, et al. Acta Otolaryngol Suppl. 2002;(547):113-7. doi: 10.1080/000164802760057725. Acta Otolaryngol Suppl. 2002. PMID: 12212585
• Expression of Epstein-Barr virus-encoded RNAs as a marker for metastatic undifferentiated nasopharyngeal carcinoma.
  Chao TY, Chow KC, Chang JY, Wang CC, Tsao TY, Harn HJ, Chi KH. Chao TY, et al. Cancer. 1996 Jul 1;78(1):24-9. doi: 10.1002/(SICI)1097-0142(19960701)78:1<24::AID-CNCR5>3.0.CO;2-H. Cancer. 1996. PMID: 8646721
• Rapid in situ hybridization for the diagnosis of latent Epstein-Barr virus infection.
  Barletta JM, Kingma DW, Ling Y, Charache P, Mann RB, Ambinder RF. Barletta JM, et al. Mol Cell Probes. 1993 Apr;7(2):105-9. doi: 10.1006/mcpr.1993.1014. Mol Cell Probes. 1993. PMID: 8391639
• [Molecular pathological studies on Epstein-Barr virus (EBV) infected cells and EBV-associated tumor cells].
  Yamamoto T, Hirai K. Yamamoto T, et al. Nihon Rinsho. 1997 Feb;55(2):452-60. Nihon Rinsho. 1997. PMID: 9046840 Review. Japanese.

Cited by

• Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma.
  Chen Y, Zhang Y, Duo S, Liu W, Luo B. Chen Y, et al. Virus Genes. 2024 Aug;60(4):347-356. doi: 10.1007/s11262-024-02071-w. Epub 2024 May 13. Virus Genes. 2024. PMID: 38739247
• Epstein-Barr Virus Regulates Endothelin-1 Expression through the ERK/FOXO1 Pathway in EBV-Associated Gastric Cancer.
  Liu W, Zhang Q, Zhang Y, Sun L, Xiao H, Luo B. Liu W, et al. Microbiol Spectr. 2023 Feb 14;11(1):e0089822. doi: 10.1128/spectrum.00898-22. Epub 2022 Dec 8. Microbiol Spectr. 2023. PMID: 36475746 Free PMC article.
• A deep learning model and human-machine fusion for prediction of EBV-associated gastric cancer from histopathology.
  Zheng X, Wang R, Zhang X, Sun Y, Zhang H, Zhao Z, Zheng Y, Luo J, Zhang J, Wu H, Huang D, Zhu W, Chen J, Cao Q, Zeng H, Luo R, Li P, Lan L, Yun J, Xie D, Zheng WS, Luo J, Cai M. Zheng X, et al. Nat Commun. 2022 May 19;13(1):2790. doi: 10.1038/s41467-022-30459-5. Nat Commun. 2022. PMID: 35589792 Free PMC article.
• Epstein-Barr Virus-Associated Malignancies and Immune Escape: The Role of the Tumor Microenvironment and Tumor Cell Evasion Strategies.
  Bauer M, Jasinski-Bergner S, Mandelboim O, Wickenhauser C, Seliger B. Bauer M, et al. Cancers (Basel). 2021 Oct 16;13(20):5189. doi: 10.3390/cancers13205189. Cancers (Basel). 2021. PMID: 34680337 Free PMC article. Review.
• Reappraisal of the incidence, various types and risk factors of malignancies in patients with dermatomyositis and polymyositis in Taiwan.
  Hsu JL, Liao MF, Chu CC, Kuo HC, Lyu RK, Chang HS, Chen CM, Wu YR, Chang KH, Weng YC, Chang CW, Chiang HI, Cheng CK, Lee PW, Huang CC, Ro LS. Hsu JL, et al. Sci Rep. 2021 Feb 25;11(1):4545. doi: 10.1038/s41598-021-83729-5. Sci Rep. 2021. PMID: 33633147 Free PMC article.

References

1. 1. J Virol. 1988 Mar;62(3):902-11 - PubMed
2. 1. Nature. 1973 Mar 2;242(5392):44-7 - PubMed
3. 1. J Gen Virol. 1988 May;69 ( Pt 5):1051-65 - PubMed
4. 1. Ann Intern Med. 1986 Mar;104(3):331-7 - PubMed
5. 1. Lancet. 1986 Nov 15;2(8516):1122-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central