Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity - PubMed (original) (raw)
. 2006 Apr 14;281(15):10355-64.
doi: 10.1074/jbc.M511314200. Epub 2006 Feb 15.
Affiliations
- PMID: 16481321
- DOI: 10.1074/jbc.M511314200
Free article
Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity
Hiroko Tanaka et al. J Biol Chem. 2006.
Free article
Abstract
The Rho family of small GTPases has been implicated in the reorganization of actin cytoskeleton and subsequent morphological changes in various cells. Rnd2 is a member of the Rnd subfamily, comprising Rnd1, Rnd2, and Rnd3. In contrast to Rnd1 and Rnd3, displaying an antagonistic action for RhoA signaling, signaling pathways of Rnd2 are not well known. Here we have performed a yeast two-hybrid screen using Rnd2 as bait and identified a novel Rnd2 effector protein, predominantly expressed in neurons, including cortical and hippocampal neurons. We named it Pragmin (pragma of Rnd2). In in vivo and in vitro binding assays, Pragmin specifically binds to Rnd2 among the Rho family GTPases in a GTP-dependent manner. Rnd2-bound Pragmin significantly stimulates RhoA activity and induces cell contraction through RhoA and the Rho-kinase pathway in HeLa cells. In PC12 cells, expressing Pragmin inhibits nerve growth factor-induced neurite outgrowth in response to Rnd2, and knock-down of Pragmin by Pragmin-specific small interfering RNA enhances neurite elongation. Therefore, Rnd2 regulates neurite outgrowth by functioning as the RhoA activator through Pragmin, in contrast to Rnd1 and Rnd3 inhibiting RhoA signaling.
Similar articles
- Rapostlin is a novel effector of Rnd2 GTPase inducing neurite branching.
Fujita H, Katoh H, Ishikawa Y, Mori K, Negishi M. Fujita H, et al. J Biol Chem. 2002 Nov 22;277(47):45428-34. doi: 10.1074/jbc.M208090200. Epub 2002 Sep 18. J Biol Chem. 2002. PMID: 12244061 - The Semaphorin 4D receptor Plexin-B1 is a GTPase activating protein for R-Ras.
Oinuma I, Ishikawa Y, Katoh H, Negishi M. Oinuma I, et al. Science. 2004 Aug 6;305(5685):862-5. doi: 10.1126/science.1097545. Science. 2004. PMID: 15297673 - Function and regulation of RhoE.
Riento K, Villalonga P, Garg R, Ridley A. Riento K, et al. Biochem Soc Trans. 2005 Aug;33(Pt 4):649-51. doi: 10.1042/BST0330649. Biochem Soc Trans. 2005. PMID: 16042565 Review. - Pathophysiological functions of Rnd proteins.
Basbous S, Azzarelli R, Pacary E, Moreau V. Basbous S, et al. Small GTPases. 2021 Sep-Nov;12(5-6):336-357. doi: 10.1080/21541248.2020.1829914. Epub 2020 Oct 15. Small GTPases. 2021. PMID: 33054516 Free PMC article. Review.
Cited by
- Molecular mechanisms of corpus callosum development: a four-step journey.
Gavrish M, Kustova A, Celis Suescún JC, Bessa P, Mitina N, Tarabykin V. Gavrish M, et al. Front Neuroanat. 2024 Jan 17;17:1276325. doi: 10.3389/fnana.2023.1276325. eCollection 2023. Front Neuroanat. 2024. PMID: 38298831 Free PMC article. Review. - Dynamic fusion of genomics and functional network connectivity in UK biobank reveals static and time-varying SNP manifolds.
Chen J, Iraji A, Fu Z, Andrés-Camazón P, Thapaliya B, Liu J, Calhoun VD. Chen J, et al. medRxiv [Preprint]. 2024 Jan 9:2024.01.09.24301013. doi: 10.1101/2024.01.09.24301013. medRxiv. 2024. PMID: 38260328 Free PMC article. Preprint. - Investigating the Protective Effects of a Citrus Flavonoid on the Retardation Morphogenesis of the Oligodendroglia-like Cell Line by Rnd2 Knockdown.
Fukatsu S, Miyamoto Y, Oka Y, Ishibashi M, Shirai R, Ishida Y, Endo S, Katoh H, Yamauchi J. Fukatsu S, et al. Neurol Int. 2023 Dec 26;16(1):33-61. doi: 10.3390/neurolint16010003. Neurol Int. 2023. PMID: 38251051 Free PMC article. - Molecular basis and current insights of atypical Rho small GTPase in cancer.
Huang H, Wang S, Guan Y, Ren J, Liu X. Huang H, et al. Mol Biol Rep. 2024 Jan 18;51(1):141. doi: 10.1007/s11033-023-09140-7. Mol Biol Rep. 2024. PMID: 38236467 Review. - Candidate Genes for IgA Nephropathy in Pediatric Patients: Exome-Wide Association Study.
Buianova AA, Proskura MV, Cheranev VV, Belova VA, Shmitko AO, Pavlova AS, Vasiliadis IA, Suchalko ON, Rebrikov DV, Petrosyan EK, Korostin DO. Buianova AA, et al. Int J Mol Sci. 2023 Nov 5;24(21):15984. doi: 10.3390/ijms242115984. Int J Mol Sci. 2023. PMID: 37958966 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases