KiSS-1 neurones are direct targets for leptin in the ob/ob mouse - PubMed (original) (raw)
Comparative Study
KiSS-1 neurones are direct targets for leptin in the ob/ob mouse
J T Smith et al. J Neuroendocrinol. 2006 Apr.
Abstract
Leptin is an adipocyte-derived hormone that acts on the hypothalamus to influence feeding, metabolism and reproduction, but the cellular and molecular targets for the action of leptin in the brain have yet to be fully elucidated. Kisspeptins are encoded by the Kiss1 gene, which is expressed in the hypothalamus and has been implicated in the neuroendocrine regulation of gonadotrophin-releasing hormone secretion. We tested the hypothesis that kisspeptin-expressing neurones are targets for leptin. First, we examined whether leptin regulates the expression of Kiss1 by comparing levels of KiSS-1 mRNA in the arcuate nucleus among groups of mice having different circulating levels of leptin: (i) wild-type (WT); (ii) leptin-deficient ob/ob; and (iii) ob/ob mice treated with leptin. All mice were castrated to control for endogenous concentrations of gonadal steroids. KiSS-1 mRNA was significantly reduced in ob/ob compared to WT mice and levels of KiSS-1 mRNA in ob/ob mice treated with leptin were increased, but not fully restored to that found in WT animals. Second, we performed double-label in situ hybridisation for KiSS-1 mRNA and the leptin receptor (Ob-Rb) mRNA and found that almost one-half (approximately 40%) of KiSS-1 mRNA-expressing cells in the arcuate nucleus expressed Ob-Rb mRNA. These results demonstrate that KiSS-1 neurones are direct targets for regulation by leptin and suggest that the reproductive deficits associated with leptin-deficient states may be attributable, in part, to diminished expression of Kiss1.
Similar articles
- Changes in expression of the genes for the leptin receptor and the growth hormone-releasing peptide/ghrelin receptor in the hypothalamic arcuate nucleus with long-term manipulation of adiposity by dietary means.
Kurose Y, Iqbal J, Rao A, Murata Y, Hasegawa Y, Terashima Y, Kojima M, Kangawa K, Clarke IJ. Kurose Y, et al. J Neuroendocrinol. 2005 Jun;17(6):331-40. doi: 10.1111/j.1365-2826.2005.01318.x. J Neuroendocrinol. 2005. PMID: 15929739 - Stereological analysis of estrogen receptor expression in the hypothalamic arcuate nucleus of ob/ob and agouti mice.
Chakraborty S, Sachdev A, Salton SR, Chakraborty TR. Chakraborty S, et al. Brain Res. 2008 Jun 27;1217:86-95. doi: 10.1016/j.brainres.2008.04.031. Epub 2008 Apr 23. Brain Res. 2008. PMID: 18502406 - Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice.
Luque RM, Huang ZH, Shah B, Mazzone T, Kineman RD. Luque RM, et al. Am J Physiol Endocrinol Metab. 2007 Mar;292(3):E891-9. doi: 10.1152/ajpendo.00258.2006. Epub 2006 Nov 22. Am J Physiol Endocrinol Metab. 2007. PMID: 17122091 - KiSS-1 and reproduction: focus on its role in the metabolic regulation of fertility.
Tena-Sempere M. Tena-Sempere M. Neuroendocrinology. 2006;83(5-6):275-81. doi: 10.1159/000095549. Epub 2006 Aug 29. Neuroendocrinology. 2006. PMID: 16940711 Review. - Synaptic plasticity in energy balance regulation.
Horvath TL. Horvath TL. Obesity (Silver Spring). 2006 Aug;14 Suppl 5:228S-233S. doi: 10.1038/oby.2006.314. Obesity (Silver Spring). 2006. PMID: 17021372 Review.
Cited by
- IGF-1 Acts through Kiss1-expressing Cells to Influence Metabolism and Reproduction.
Wang M, Pugh SM, Daboul J, Miller D, Xu Y, Hill JW. Wang M, et al. bioRxiv [Preprint]. 2024 Jul 4:2024.07.02.601722. doi: 10.1101/2024.07.02.601722. bioRxiv. 2024. PMID: 39005405 Free PMC article. Preprint. - The influence of body composition on the response to dynamic stimulation of the endocrine pituitary-testis axis.
Abildgaard J, Bang AK, Nordkap L, Priskorn L, Jørgensen N. Abildgaard J, et al. Int J Obes (Lond). 2024 Sep;48(9):1216-1222. doi: 10.1038/s41366-024-01518-2. Epub 2024 Apr 12. Int J Obes (Lond). 2024. PMID: 38609526 Free PMC article. - Mapping the Landscape of Obesity Effects on Male Reproductive Function: A Bibliometric Study.
Wei Y, Liao M, Lu Y, Lei X, Wang J, Luo X, Hu L. Wei Y, et al. Endocr Metab Immune Disord Drug Targets. 2024;24(13):1546-1557. doi: 10.2174/0118715303271117231220072051. Endocr Metab Immune Disord Drug Targets. 2024. PMID: 38299282 - Physical, psychological and biochemical recovery from anabolic steroid-induced hypogonadism: a scoping review.
Solanki P, Eu B, Smith J, Allan C, Lee K. Solanki P, et al. Endocr Connect. 2023 Oct 19;12(12):e230358. doi: 10.1530/EC-23-0358. Print 2023 Dec 1. Endocr Connect. 2023. PMID: 37855241 Free PMC article. - Alterations in Appetite-Regulating Hormones in Girls with Central Early or Precocious Puberty.
Stathori G, Tzounakou AM, Mastorakos G, Vlahos NF, Charmandari E, Valsamakis G. Stathori G, et al. Nutrients. 2023 Oct 9;15(19):4306. doi: 10.3390/nu15194306. Nutrients. 2023. PMID: 37836591 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous