The lack of effect of glucosamine sulphate on aggrecan mRNA expression and (35)S-sulphate incorporation in bovine primary chondrocytes - PubMed (original) (raw)
. 2006 Apr;1762(4):453-9.
doi: 10.1016/j.bbadis.2006.01.005. Epub 2006 Feb 10.
Affiliations
- PMID: 16504489
- DOI: 10.1016/j.bbadis.2006.01.005
Free article
The lack of effect of glucosamine sulphate on aggrecan mRNA expression and (35)S-sulphate incorporation in bovine primary chondrocytes
Cheng-Juan Qu et al. Biochim Biophys Acta. 2006 Apr.
Free article
Abstract
Glucosamine and glucosamine sulphate have been promoted as a disease-modifying agent to improve the clinical symptoms of osteoarthritis. The precise mechanism of the action of the suggested positive effect of glucosamine or glucosamine sulphate on cartilage proteoglycans is not known, since the level of glucosamine in plasma remains very low after oral administration of glucosamine sulphate. We examined whether exogenous hexosamines or their sulphated forms would increase steady-state levels of aggrecan and hyaluronan synthase (HAS) or glycosaminoglycan synthesis using Northern blot and (35)S-sulphate incorporation analyses. Total RNA was extracted from bovine primary chondrocytes which were cultured either in 1 mM concentration of glucosamine, galactosamine, mannosamine, glucosamine 3-sulphate, glucosamine 6-sulphate or galactosamine 6-sulphate for 0, 4, 8 and 24 h, or in three different concentrations (control, 100 microM and 1 mM) of glucosamine sulphate salt or glucose for 24 or 72 h. Northern blot assay showed that neither hexosamines nor glucosamine sulphate salt stimulated aggrecan and HAS-2 mRNA expression. Glycosaminoglycan synthesis remained at a control level in the treated cultures, with the exception of mannosamine which inhibited (35)S-sulphate incorporation in low-glucose DMEM treatment. In our culture conditions, hexosamines or their sulphated forms did not increase aggrecan expression or (35)S-sulphate incorporation.
Similar articles
- Glucosamine sulphate does not increase extracellular matrix production at low oxygen tension.
Qu CJ, Pöytäkangas T, Jauhiainen M, Auriola S, Lammi MJ. Qu CJ, et al. Cell Tissue Res. 2009 Jul;337(1):103-11. doi: 10.1007/s00441-009-0797-7. Epub 2009 May 14. Cell Tissue Res. 2009. PMID: 19440735 - Expression of reduced amounts of structurally altered aggrecan in articular cartilage chondrocytes exposed to high hydrostatic pressure.
Lammi MJ, Inkinen R, Parkkinen JJ, Häkkinen T, Jortikka M, Nelimarkka LO, Järveläinen HT, Tammi MI. Lammi MJ, et al. Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):723-30. doi: 10.1042/bj3040723. Biochem J. 1994. PMID: 7818473 Free PMC article. - Effects of long-term exposure to glucosamine and mannosamine on aggrecan degradation in articular cartilage.
Ilic MZ, Martinac B, Handley CJ. Ilic MZ, et al. Osteoarthritis Cartilage. 2003 Aug;11(8):613-22. doi: 10.1016/s1063-4584(03)00092-x. Osteoarthritis Cartilage. 2003. PMID: 12880584 - Glucosamine decreases expression of anabolic and catabolic genes in human osteoarthritic cartilage explants.
Uitterlinden EJ, Jahr H, Koevoet JL, Jenniskens YM, Bierma-Zeinstra SM, Degroot J, Verhaar JA, Weinans H, van Osch GJ. Uitterlinden EJ, et al. Osteoarthritis Cartilage. 2006 Mar;14(3):250-7. doi: 10.1016/j.joca.2005.10.001. Epub 2005 Nov 18. Osteoarthritis Cartilage. 2006. PMID: 16300972 - Effects of interferon-gamma and tumor necrosis factor alpha on the expression of the genes encoding aggrecan, biglycan, and decorin core proteins in cultured human chondrocytes.
Dodge GR, Diaz A, Sanz-Rodriguez C, Reginato AM, Jimenez SA. Dodge GR, et al. Arthritis Rheum. 1998 Feb;41(2):274-83. doi: 10.1002/1529-0131(199802)41:2<274::AID-ART11>3.0.CO;2-Z. Arthritis Rheum. 1998. PMID: 9485085 Review.
Cited by
- Transdermal co-delivery of glucosamine sulfate and diacerein for the induction of chondroprotection in experimental osteoarthritis.
Chattopadhyay H, Auddy B, Sur T, Gupta M, Datta S. Chattopadhyay H, et al. Drug Deliv Transl Res. 2020 Oct;10(5):1327-1340. doi: 10.1007/s13346-019-00701-7. Drug Deliv Transl Res. 2020. PMID: 31907788 - High glycine concentration increases collagen synthesis by articular chondrocytes in vitro: acute glycine deficiency could be an important cause of osteoarthritis.
de Paz-Lugo P, Lupiáñez JA, Meléndez-Hevia E. de Paz-Lugo P, et al. Amino Acids. 2018 Oct;50(10):1357-1365. doi: 10.1007/s00726-018-2611-x. Epub 2018 Jul 13. Amino Acids. 2018. PMID: 30006659 Free PMC article. - Rapid Myoglobin Aggregation through Glucosamine-Induced α-Dicarbonyl Formation.
Hrynets Y, Ndagijimana M, Betti M. Hrynets Y, et al. PLoS One. 2015 Sep 25;10(9):e0139022. doi: 10.1371/journal.pone.0139022. eCollection 2015. PLoS One. 2015. PMID: 26406447 Free PMC article. - Differential metabolic effects of glucosamine and N-acetylglucosamine in human articular chondrocytes.
Shikhman AR, Brinson DC, Valbracht J, Lotz MK. Shikhman AR, et al. Osteoarthritis Cartilage. 2009 Aug;17(8):1022-8. doi: 10.1016/j.joca.2009.03.004. Epub 2009 Mar 24. Osteoarthritis Cartilage. 2009. PMID: 19332174 Free PMC article. - Dietary glucosamine under question.
Silbert JE. Silbert JE. Glycobiology. 2009 Jun;19(6):564-7. doi: 10.1093/glycob/cwp014. Epub 2009 Mar 2. Glycobiology. 2009. PMID: 19254962 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources