Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus - PubMed (original) (raw)

Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus

M W Bauer et al. Appl Environ Microbiol. 1997 Mar.

Abstract

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

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