Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila - PubMed (original) (raw)
. 2006 Mar 17;21(6):811-23.
doi: 10.1016/j.molcel.2006.02.007.
Mikko Taipale, Jop Kind, Herbert Holz, Philipp Gebhardt, Malgorzata Schelder, Michiel Vermeulen, Alessia Buscaino, Kent Duncan, Juerg Mueller, Matthias Wilm, Henk G Stunnenberg, Harald Saumweber, Asifa Akhtar
Affiliations
- PMID: 16543150
- DOI: 10.1016/j.molcel.2006.02.007
Free article
Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila
Sascha Mendjan et al. Mol Cell. 2006.
Free article
Abstract
Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.
Similar articles
- MOF-regulated acetylation of MSL-3 in the Drosophila dosage compensation complex.
Buscaino A, Köcher T, Kind JH, Holz H, Taipale M, Wagner K, Wilm M, Akhtar A. Buscaino A, et al. Mol Cell. 2003 May;11(5):1265-77. doi: 10.1016/s1097-2765(03)00140-0. Mol Cell. 2003. PMID: 12769850 - The right dose for every sex.
Mendjan S, Akhtar A. Mendjan S, et al. Chromosoma. 2007 Apr;116(2):95-106. doi: 10.1007/s00412-006-0089-x. Epub 2006 Nov 24. Chromosoma. 2007. PMID: 17124606 Free PMC article. Review. - Correct dosage of X chromosome transcription is controlled by a nuclear pore component.
Aleman JR, Kuhn TM, Pascual-Garcia P, Gospocic J, Lan Y, Bonasio R, Little SC, Capelson M. Aleman JR, et al. Cell Rep. 2021 Jun 15;35(11):109236. doi: 10.1016/j.celrep.2021.109236. Cell Rep. 2021. PMID: 34133927 Free PMC article. - A new strategy for isolating genes controlling dosage compensation in Drosophila using a simple epigenetic mosaic eye phenotype.
Prabhakaran M, Kelley RL. Prabhakaran M, et al. BMC Biol. 2010 Jun 10;8:80. doi: 10.1186/1741-7007-8-80. BMC Biol. 2010. PMID: 20537125 Free PMC article. - Dosage compensation and chromatin structure in Drosophila.
Bashaw GJ, Baker BS. Bashaw GJ, et al. Curr Opin Genet Dev. 1996 Aug;6(4):496-501. doi: 10.1016/s0959-437x(96)80073-6. Curr Opin Genet Dev. 1996. PMID: 8791531 Review.
Cited by
- Basilicata-Akhtar Syndrome: Unraveling an Ultrarare Cause of Developmental Delay.
Kulkarni V, Chalipat S, Gupta A, Bhosle A, Bahal M. Kulkarni V, et al. Cureus. 2024 Aug 16;16(8):e67041. doi: 10.7759/cureus.67041. eCollection 2024 Aug. Cureus. 2024. PMID: 39286690 Free PMC article. - Lysine Methylation-Dependent Proteolysis by the Malignant Brain Tumor (MBT) Domain Proteins.
Sun H, Zhang H. Sun H, et al. Int J Mol Sci. 2024 Feb 13;25(4):2248. doi: 10.3390/ijms25042248. Int J Mol Sci. 2024. PMID: 38396925 Free PMC article. Review. - Stabilization of MOF (KAT8) by USP10 promotes esophageal squamous cell carcinoma proliferation and metastasis through epigenetic activation of ANXA2/Wnt signaling.
Li P, Yang L, Park SY, Liu F, Li AH, Zhu Y, Sui H, Gao F, Li L, Ye L, Zou Y, Tian Z, Zhao Y, Costa M, Sun H, Zhao X. Li P, et al. Oncogene. 2024 Mar;43(12):899-917. doi: 10.1038/s41388-024-02955-z. Epub 2024 Feb 5. Oncogene. 2024. PMID: 38317006 - Hotspot Cancer Mutation Impairs KAT8-mediated Nucleosomal Histone Acetylation.
Xuan H, Xu L, Li K, Xuan F, Xu T, Wen H, Shi X. Xuan H, et al. J Mol Biol. 2024 Apr 1;436(7):168413. doi: 10.1016/j.jmb.2023.168413. Epub 2023 Dec 20. J Mol Biol. 2024. PMID: 38135180 - Comprehensive synergy mapping links a BAF- and NSL-containing "supercomplex" to the transcriptional silencing of HIV-1.
Li Z, Deeks SG, Ott M, Greene WC. Li Z, et al. Cell Rep. 2023 Sep 26;42(9):113055. doi: 10.1016/j.celrep.2023.113055. Epub 2023 Sep 7. Cell Rep. 2023. PMID: 37682714 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous