Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor - PubMed (original) (raw)

Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor

L Naldini et al. EMBO J. 1991 Oct.

Abstract

Scatter Factor (SF) is a fibroblast-secreted protein which promotes motility and matrix invasion of epithelial cells. Hepatocyte Growth Factor (HGF) is a powerful mitogen for hepatocytes and other epithelial tissues. SF and HGF, purified according to their respective biological activities, were interchangeable and equally effective in assays for cell growth, motility and invasion. Both bound with identical affinities to the same sites in target cells. The receptor for SF and HGF was identified as the product of the MET oncogene by: (i) ligand binding and coprecipitation in immunocomplexes; (ii) chemical crosslinking to the Met beta subunit; (iii) transfer of binding activity in insect cells by a baculovirus carrying the MET cDNA; (iv) ligand-induced tyrosine phosphorylation of the Met beta subunit. SF and HGF cDNA clones from human fibroblasts, placenta and liver had virtually identical sequences. We conclude that the same molecule (SF/HGF) acts as a growth or motility factor through a single receptor in different target cells.

PubMed Disclaimer

Similar articles

• Molecular characteristics of HGF-SF and its role in cell motility and invasion.
  Weidner KM, Hartmann G, Naldini L, Comoglio PM, Sachs M, Fonatsch C, Rieder H, Birchmeier W. Weidner KM, et al. EXS. 1993;65:311-28. EXS. 1993. PMID: 8380739 Review.
• A functional domain in the heavy chain of scatter factor/hepatocyte growth factor binds the c-Met receptor and induces cell dissociation but not mitogenesis.
  Hartmann G, Naldini L, Weidner KM, Sachs M, Vigna E, Comoglio PM, Birchmeier W. Hartmann G, et al. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11574-8. doi: 10.1073/pnas.89.23.11574. Proc Natl Acad Sci U S A. 1992. PMID: 1280830 Free PMC article.
• The Met receptor tyrosine kinase transduces motility, proliferation, and morphogenic signals of scatter factor/hepatocyte growth factor in epithelial cells.
  Weidner KM, Sachs M, Birchmeier W. Weidner KM, et al. J Cell Biol. 1993 Apr;121(1):145-54. doi: 10.1083/jcb.121.1.145. J Cell Biol. 1993. PMID: 8384622 Free PMC article.
• Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product.
  Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA. Bottaro DP, et al. Science. 1991 Feb 15;251(4995):802-4. doi: 10.1126/science.1846706. Science. 1991. PMID: 1846706

Cited by

• The MET Oncogene: Thirty Years of Insights into Molecular Mechanisms Driving Malignancy.
  Crepaldi T, Gallo S, Comoglio PM. Crepaldi T, et al. Pharmaceuticals (Basel). 2024 Mar 30;17(4):448. doi: 10.3390/ph17040448. Pharmaceuticals (Basel). 2024. PMID: 38675409 Free PMC article.
• Oncogenic STAT Transcription Factors as Targets for Cancer Therapy: Innovative Strategies and Clinical Translation.
  Wang W, Lopez McDonald MC, Hariprasad R, Hamilton T, Frank DA. Wang W, et al. Cancers (Basel). 2024 Mar 31;16(7):1387. doi: 10.3390/cancers16071387. Cancers (Basel). 2024. PMID: 38611065 Free PMC article. Review.
• Knockout of c-Cbl/Cbl-b slows c-Met trafficking resulting in enhanced signaling in corneal epithelial cells.
  Tarvestad-Laise K, Ceresa BP. Tarvestad-Laise K, et al. J Biol Chem. 2023 Oct;299(10):105233. doi: 10.1016/j.jbc.2023.105233. Epub 2023 Sep 9. J Biol Chem. 2023. PMID: 37690689 Free PMC article.
• Downstream Targets of VHL/HIF-α Signaling in Renal Clear Cell Carcinoma Progression: Mechanisms and Therapeutic Relevance.
  Mazumder S, Higgins PJ, Samarakoon R. Mazumder S, et al. Cancers (Basel). 2023 Feb 19;15(4):1316. doi: 10.3390/cancers15041316. Cancers (Basel). 2023. PMID: 36831657 Free PMC article. Review.
• Reduced HGF/MET Signaling May Contribute to the Synaptic Pathology in an Alzheimer's Disease Mouse Model.
  Wei J, Ma X, Nehme A, Cui Y, Zhang L, Qiu S. Wei J, et al. Front Aging Neurosci. 2022 Jul 12;14:954266. doi: 10.3389/fnagi.2022.954266. eCollection 2022. Front Aging Neurosci. 2022. PMID: 35903536 Free PMC article.

References

1. 1. Oncogene. 1991 Apr;6(4):553-9 - PubMed
2. 1. Nature. 1989 Nov 23;342(6248):440-3 - PubMed
3. 1. Cell. 1991 Apr 5;65(1):189-97 - PubMed
4. 1. Mol Cell Biol. 1991 Jun;11(6):2962-70 - PubMed
5. 1. Cell Growth Differ. 1990 Feb;1(2):87-95 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations

• Miscellaneous

  • NCI CPTAC Assay Portal