Convergent transcription initiates from oppositely oriented promoters within the 5' end regions of Drosophila melanogaster F elements - PubMed (original) (raw)

Convergent transcription initiates from oppositely oriented promoters within the 5' end regions of Drosophila melanogaster F elements

G Minchiotti et al. Mol Cell Biol. 1991 Oct.

Abstract

Drosophila melanogaster F elements are mobile, oligo(A)-terminated DNA sequences that likely propagate by the retrotranscription of RNA intermediates. Plasmids bearing DNA segments from the left-hand region of a full-length F element fused to the CAT gene were used as templates for transient expression assays in Drosophila Schneider II cultured cells. Protein and RNA analyses led to the identification of two promoters, Fin and Fout, that transcribe in opposite orientations. The Fin promoter drives the synthesis of transcripts that initiate around residue +6 and are directed toward the element. Fin, that probably controls the formation of F transposition RNA intermediates and gene products, is internal to the transcribed region. Sequences important for accumulation of Fin transcripts are included within the +1 to +30 interval; an additional regulatory element may coincide with a heptamer located downstream of this region also found in the 5' end regions of F-like Drosophila retrotransposons. Analysis of the template activity of 3' deletion derivatives indicates that the level of accumulation of Fin RNA is also dependent upon the presence of sequences located within the +175 to +218 interval. The Fout promoter drives transcription in the opposite orientation with respect to Fin. Fout transcripts initiate at nearby sites within the +92 to +102 interval. Sequences downstream of these multiple RNA start sites are not required for the activity of the Fout promoter. Deletions knocking out the Fin promoter do not impair Fout transcription; conversely, initiation at the Fin promoter still takes place in templates that lack the Fout promoter. At a low level, both promoters are active in cultured cells.

PubMed Disclaimer

Cited by

Distinct families of site-specific retrotransposons occupy identical positions in the rRNA genes of Anopheles gambiae.
Besansky NJ, Paskewitz SM, Hamm DM, Collins FH. Besansky NJ, et al. Mol Cell Biol. 1992 Nov;12(11):5102-10. doi: 10.1128/mcb.12.11.5102-5110.1992. Mol Cell Biol. 1992. PMID: 1328871 Free PMC article.
Euchromatic transposon insertions trigger production of novel Pi- and endo-siRNAs at the target sites in the drosophila germline.
Shpiz S, Ryazansky S, Olovnikov I, Abramov Y, Kalmykova A. Shpiz S, et al. PLoS Genet. 2014 Feb 6;10(2):e1004138. doi: 10.1371/journal.pgen.1004138. eCollection 2014 Feb. PLoS Genet. 2014. PMID: 24516406 Free PMC article.
Downstream Antisense Transcription Predicts Genomic Features That Define the Specific Chromatin Environment at Mammalian Promoters.
Lavender CA, Cannady KR, Hoffman JA, Trotter KW, Gilchrist DA, Bennett BD, Burkholder AB, Burd CJ, Fargo DC, Archer TK. Lavender CA, et al. PLoS Genet. 2016 Aug 3;12(8):e1006224. doi: 10.1371/journal.pgen.1006224. eCollection 2016 Aug. PLoS Genet. 2016. PMID: 27487356 Free PMC article.
Non-long terminal repeat (non-LTR) retrotransposons: mechanisms, recent developments, and unanswered questions.
Han JS. Han JS. Mob DNA. 2010 May 12;1(1):15. doi: 10.1186/1759-8753-1-15. Mob DNA. 2010. PMID: 20462415 Free PMC article.
Expression of Drosophila melanogaster F elements in vivo.
Minchiotti G, Contursi C, Graziani F, Gargiulo G, Di Nocera PP. Minchiotti G, et al. Mol Gen Genet. 1994 Oct 28;245(2):152-9. doi: 10.1007/BF00283262. Mol Gen Genet. 1994. PMID: 7816022

References

1. Genomics. 1991 Apr;9(4):728-36 - PubMed
1. Nature. 1986 Jun 5-11;321(6070):625-8 - PubMed
1. Nucleic Acids Res. 1986 Jan 24;14(2):623-33 - PubMed
1. Annu Rev Biochem. 1986;55:631-61 - PubMed
1. Cell. 1986 Dec 26;47(6):1007-15 - PubMed

Convergent transcription initiates from oppositely oriented promoters within the 5' end regions of Drosophila melanogaster F elements - PubMed (original) (raw)