Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence - PubMed (original) (raw)
Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence
M D Ryan et al. J Gen Virol. 1991 Nov.
Abstract
The 2A region of the foot-and-mouth disease virus (FMDV) polyprotein is only 16 amino acids in length. During synthesis of the FMDV polyprotein a primary proteolytic processing event occurs between the 2A and 2B regions of the polyprotein. The activity responsible for this cleavage is not known but it is thought that either an unidentified virus-encoded proteinase may be responsible, or that 2A acts as a substrate for a host cell proteinase. A series of recombinant FMDV polyproteins has been constructed in which sequences to the N- or C-terminal side of the 2A region have been deleted. Analysis of the processing of these polyproteins shows that a 19 amino acid sequence spanning 2A is sufficient to mediate polyprotein cleavage at a site immediately C-terminal to 2A, whereas deletions extending into the 2A region prevent cleavage.
Similar articles
- The cleavage activities of aphthovirus and cardiovirus 2A proteins.
Donnelly ML, Gani D, Flint M, Monaghan S, Ryan MD. Donnelly ML, et al. J Gen Virol. 1997 Jan;78 ( Pt 1):13-21. doi: 10.1099/0022-1317-78-1-13. J Gen Virol. 1997. PMID: 9010280 - Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'.
Donnelly MLL, Luke G, Mehrotra A, Li X, Hughes LE, Gani D, Ryan MD. Donnelly MLL, et al. J Gen Virol. 2001 May;82(Pt 5):1013-1025. doi: 10.1099/0022-1317-82-5-1013. J Gen Virol. 2001. PMID: 11297676 - Specificity of enzyme-substrate interactions in foot-and-mouth disease virus polyprotein processing.
Ryan MD, Belsham GJ, King AM. Ryan MD, et al. Virology. 1989 Nov;173(1):35-45. doi: 10.1016/0042-6822(89)90219-5. Virology. 1989. PMID: 2554577 - Hepatitis A virus proteins.
Totsuka A, Moritsugu Y. Totsuka A, et al. Intervirology. 1999;42(2-3):63-8. doi: 10.1159/000024967. Intervirology. 1999. PMID: 10516462 Review. - Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure.
Belsham GJ. Belsham GJ. Prog Biophys Mol Biol. 1993;60(3):241-60. doi: 10.1016/0079-6107(93)90016-d. Prog Biophys Mol Biol. 1993. PMID: 8396787 Free PMC article. Review. No abstract available.
Cited by
- Visualization, Fate Mapping, Ablation, and Mutagenesis of Microglia in the Mouse Brain.
Kim JS, Jung S. Kim JS, et al. Adv Neurobiol. 2024;37:53-63. doi: 10.1007/978-3-031-55529-9_4. Adv Neurobiol. 2024. PMID: 39207686 - A fluorescence-based sensor for calibrated measurement of protein kinase stability in live cells.
Paul JW 3rd, Muratcioğlu S, Kuriyan J. Paul JW 3rd, et al. Protein Sci. 2024 Jun;33(6):e5023. doi: 10.1002/pro.5023. Protein Sci. 2024. PMID: 38801214 Free PMC article. - Development of a compact bidirectional promoter-driven dual chimeric antigen receptor (CAR) construct targeting CD19 and CD20 in the Sleeping Beauty (SB) transposon system.
Khaniya A, Rad SMAH, Halpin J, Tawinwung S, McLellan A, Suppipat K, Hirankarn N. Khaniya A, et al. J Immunother Cancer. 2024 Apr 27;12(4):e008555. doi: 10.1136/jitc-2023-008555. J Immunother Cancer. 2024. PMID: 38677881 Free PMC article. - Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using "2A" Self-Cleaving Peptides.
Spatola Rossi T, Fricker M, Kriechbaumer V. Spatola Rossi T, et al. Methods Mol Biol. 2024;2772:337-351. doi: 10.1007/978-1-0716-3710-4_26. Methods Mol Biol. 2024. PMID: 38411827 - Multivalent cytomegalovirus glycoprotein B nucleoside modified mRNA vaccines did not demonstrate a greater antibody breadth.
Wang HY, Li L, Nelson CS, Barfield R, Valencia S, Chan C, Muramatsu H, Lin PJC, Pardi N, An Z, Weissman D, Permar SR. Wang HY, et al. NPJ Vaccines. 2024 Feb 20;9(1):38. doi: 10.1038/s41541-024-00821-3. NPJ Vaccines. 2024. PMID: 38378950 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources