Properties of a cell-free system for synthesis of citrus exocortis viroid - PubMed (original) (raw)
Properties of a cell-free system for synthesis of citrus exocortis viroid
R Flores et al. Proc Natl Acad Sci U S A. 1982 Oct.
Abstract
Partially purified nuclei from citrus exocortis viroid (CEV)-infected Gynura aurantiaca are able to synthesize linear and circular viroid molecules. Pretreatment of the nuclei with actinomycin D or deoxyribonuclease did not affect viroid synthesis, whereas the synthesis of other cellular RNAs was severely reduced. These observations support the essential role of CEV complementary RNA sequences in viroid replication. However, when alpha-amanitin was included in this in vitro synthesis system, CEV replication was markedly reduced by concentrations of 10 nM or greater. Taken together, these data support the proposition that viroid synthesis is catalyzed by a DNA-dependent RNA polymerase acting on a RNA template.
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References
- Nature. 1975 Aug 28;256(5520):753-6 - PubMed
- Virology. 1975 Mar;64(1):106-14 - PubMed
- Virology. 1975 Feb;63(2):421-7 - PubMed
- Virology. 1971 Jan;43(1):75-89 - PubMed
- Proc Natl Acad Sci U S A. 1978 Feb;75(2):896-900 - PubMed
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