Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release - PubMed (original) (raw)

. 2006 May 19;312(5776):1051-4.

doi: 10.1126/science.1126308. Epub 2006 Apr 13.

Carolin Wichmann, Tobias M Rasse, Wernher Fouquet, Manuela Schmidt, Andreas Schmid, Dhananjay A Wagh, Christian Pawlu, Robert R Kellner, Katrin I Willig, Stefan W Hell, Erich Buchner, Manfred Heckmann, Stephan J Sigrist

Affiliations

• PMID: 16614170
• DOI: 10.1126/science.1126308

Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release

Robert J Kittel et al. Science. 2006.

Abstract

The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.

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Comment in

• Neuroscience. Gatekeeper at the synapse.
  Atwood HL. Atwood HL. Science. 2006 May 19;312(5776):1008-9. doi: 10.1126/science.1128445. Science. 2006. PMID: 16709774 No abstract available.

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