Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging - PubMed (original) (raw)
Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
Emre Yaksi et al. Nat Methods. 2006 May.
Abstract
Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.
Comment in
- Imaging activity in brain cells: deconvolution clears the haze.
Strowbridge BW. Strowbridge BW. Nat Methods. 2006 May;3(5):344-6. doi: 10.1038/nmeth0506-344. Nat Methods. 2006. PMID: 16628203 No abstract available.
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