In vivo effects of serotonergic agents on alpha-melanocyte-stimulating hormone secretion - PubMed (original) (raw)
In vivo effects of serotonergic agents on alpha-melanocyte-stimulating hormone secretion
J A Carr et al. Neuroendocrinology. 1991 Dec.
Abstract
We used an in vivo pharmacological approach to investigate the potential influence of serotonin (5-HT) on peptide release from the intermediate lobe (IL) of the rat pituitary. Plasma levels of alpha-melanocyte-stimulating hormone (alpha-MSH) as well as the ultrastructural appearance of IL cells were used as indicators of IL secretory activity. Plasma beta-endorphin levels were also measured to assess the effectiveness of 5-HT-acting drugs. Intraperitoneal administration of 5-hydroxy-L-tryptophan, the synthetic precursor of 5-HT, dramatically elevated the content of 5-HT in the neurointermediate lobe but did not alter plasma titers of alpha-MSH. Treatment with the 5-HT reuptake blocker fluoxetine elevated plasma levels of beta-endorphin but not alpha-MSH. Administration of the 5-HT1/5-HT2 agonist MK-212 produced an elevation in both plasma alpha-MSH and beta-endorphin levels. Quantitative morphometry of IL cells at the ultrastructural level revealed that MK-212 treatment selectively causes depletion of electron-lucent but not electron-dense secretory granules from the cytoplasm of IL cells. Pretreatment with the selective D2 dopamine agonist apomorphine blocked MK-212-induced release of alpha-MSH but not beta-endorphin. Our results show that manipulation of 5-HT synthesis/reuptake does not affect release of alpha-MSH, but that direct activation of 5-HT receptors with the nonselective agonist MK-212 stimulates alpha-MSH release. The ability of apomorphine to block MK-212-induced release of alpha-MSH suggests a direct antagonism between dopaminergic and serotonergic regulation of alpha-MSH release.
Similar articles
- Stress-induced peptide release from rat intermediate pituitary. An ultrastructural analysis.
Carr JA, Saland LC, Samora A, Desai S, Benevidez S. Carr JA, et al. Cell Tissue Res. 1990 Sep;261(3):589-93. doi: 10.1007/BF00313539. Cell Tissue Res. 1990. PMID: 2173975 - Dopaminergic and beta-adrenergic receptor control of alpha-melanocyte-stimulating hormone secretion during stress.
Lindley SE, Lookingland KJ, Moore KE. Lindley SE, et al. Neuroendocrinology. 1990 Jul;52(1):46-51. doi: 10.1159/000125537. Neuroendocrinology. 1990. PMID: 2168526 - Effect of serotonin on alpha-melanocyte-stimulating hormone secretion from perifused frog neurointermediate lobe: evidence for the presence of serotonin-containing cells in the frog pars intermedia.
Lamacz M, Tonon MC, Leboulenger F, Héry F, Idres S, Verhofstad AJ, Pelletier G, Vaudry H. Lamacz M, et al. J Endocrinol. 1989 Jul;122(1):135-46. doi: 10.1677/joe.0.1220135. J Endocrinol. 1989. PMID: 2549146 - Alpha melanocyte stimulating hormone (MSH) in Tourette's syndrome.
Sandyk R. Sandyk R. Int J Neurosci. 1989 Jan;44(1-2):161-4. doi: 10.3109/00207458908986193. Int J Neurosci. 1989. PMID: 2562052 Review. - Melanotrope cells as a model to understand the (patho)physiological regulation of hormone secretion.
Vàzquez-Martínez R, Peinado JR, Cruz-García D, Ruiz-Navarro A, Gracia-Navarro F, Anouar Y, Tonon MC, Vaudry H, Castaño JP, Malagón MM. Vàzquez-Martínez R, et al. J Endocrinol Invest. 2005 Nov;28(10):949-58. doi: 10.1007/BF03345330. J Endocrinol Invest. 2005. PMID: 16419501 Review.
Cited by
- Transmembrane potential of GlyCl-expressing instructor cells induces a neoplastic-like conversion of melanocytes via a serotonergic pathway.
Blackiston D, Adams DS, Lemire JM, Lobikin M, Levin M. Blackiston D, et al. Dis Model Mech. 2011 Jan;4(1):67-85. doi: 10.1242/dmm.005561. Epub 2010 Oct 19. Dis Model Mech. 2011. PMID: 20959630 Free PMC article. - The separation of exocytosis from endocytosis in rat melanotroph membrane capacitance records.
Zupancic G, Kocmur L, Veranic P, Grilc S, Kordas M, Zorec R. Zupancic G, et al. J Physiol. 1994 Nov 1;480 ( Pt 3)(Pt 3):539-52. doi: 10.1113/jphysiol.1994.sp020382. J Physiol. 1994. PMID: 7869267 Free PMC article. - Effects of serotonergic activation by 5-hydroxytryptophan on sleep and body temperature of C57BL/6J and interleukin-6-deficient mice are dose and time related.
Morrow JD, Vikraman S, Imeri L, Opp MR. Morrow JD, et al. Sleep. 2008 Jan;31(1):21-33. doi: 10.1093/sleep/31.1.21. Sleep. 2008. PMID: 18220075 Free PMC article. - Gonadotropin and prolactin secretion in prepubertal female rats treated with 8-hydroxy-2-(di-n-propylamino) tetralin.
Aguilar E, Ranchal A, Aguilar R, Pinilla L. Aguilar E, et al. J Neural Transm Gen Sect. 1993;94(3):165-73. doi: 10.1007/BF01277022. J Neural Transm Gen Sect. 1993. PMID: 7510111 - Endocytotic pathways in the melanotroph of the rat pituitary.
Bäck N, Soinila S, Virtanen I. Bäck N, et al. Histochem J. 1993 Feb;25(2):133-9. doi: 10.1007/BF00157985. Histochem J. 1993. PMID: 8385655
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources