Use of Carbon Oxysulfide, a Structural Analog of CO(2), to Study Active CO(2) Transport in the Cyanobacterium Synechococcus UTEX 625 - PubMed (original) (raw)

Use of Carbon Oxysulfide, a Structural Analog of CO(2), to Study Active CO(2) Transport in the Cyanobacterium Synechococcus UTEX 625

A G Miller et al. Plant Physiol. 1989 Jul.

Abstract

Carbon oxysulfide (carbonyl sulfide, COS) is a close structural analog of CO(2). Although hydrolysis of COS (to CO(2) and H(2)S) does occur at alkaline pH (>9), at pH 8.0 the rate of hydrolysis is slow enough to allow investigation of COS as a possible substrate and inhibitor of the active CO(2) transport system of Synechococcus UTEX 625. A light-dependent uptake of COS was observed that was inhibited by CO(2) and the ATPase inhibitor diethylstilbestrol. The COS taken up by the cells could not be recovered when the lights were turned off or when acid was added. It was concluded that most of the COS taken up was hydrolyzed by intracellular carbonic anhydrase. The production of H(2)S was observed and COS removal from the medium was inhibited by ethoxyzolamide. Bovine erythrocyte carbonic anhydrase catalysed the stoichiometric hydrolysis of COS to H(2)S. The active transport of CO(2) was inhibited by COS in an apparently competitive manner. When Na(+)-dependent HCO(3) (-) transport was allowed in the presence of COS, the extracellular [CO(2)] rose considerably above the equilibrium level. This CO(2) appearing in the medium was derived from the dehydration of transported HCO(3) (-) and was leaked from the cells. In the presence of COS the return to the cells of this leaked CO(2) was inhibited. These results showed that the Na(+)-dependent HCO(3) (-) transport was not inhibited by COS, whereas active CO(2) transport was inhibited. When COS was removed by gassing with N(2), a normal pattern of CO(2) uptake was observed. The silicone fluid centrifugation method showed that COS (100 micromolar) had little effect upon the initial rate of HCO(3) (-) transport or CO(2) fixation. The steady state rate of CO(2) fixation was, however, inhibited about 50% in the presence of COS. This inhibition can be at least partially explained by the significant leakage of CO(2) from the cells that occurred when CO(2) uptake was inhibited by COS. Neither CS(2) nor N(2)O acted like COS. It is concluded that COS is an effective and selective inhibitor of active CO(2) transport.

PubMed Disclaimer

Similar articles

• Carbon Oxysulfide Inhibition of the CO(2)-Concentrating Process of Unicellular Green Algae.
  Goyal A, Shiraiwa Y, Tolbert NE. Goyal A, et al. Plant Physiol. 1992 Feb;98(2):578-83. doi: 10.1104/pp.98.2.578. Plant Physiol. 1992. PMID: 16668680 Free PMC article.
• Carbon Oxysulfide Is an Inhibitor of Both CO(2) and HCO(3) Uptake in the Cyanobacterium Synechococcus PCC7942.
  Badger MR, Price GD. Badger MR, et al. Plant Physiol. 1990 Sep;94(1):35-9. doi: 10.1104/pp.94.1.35. Plant Physiol. 1990. PMID: 16667708 Free PMC article.
• Selective and Reversible Inhibition of Active CO(2) Transport by Hydrogen Sulfide in a Cyanobacterium.
  Espie GS, Miller AG, Canvin DT. Espie GS, et al. Plant Physiol. 1989 Sep;91(1):387-94. doi: 10.1104/pp.91.1.387. Plant Physiol. 1989. PMID: 16667030 Free PMC article.
• Active Transport of CO(2) by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry.
  Miller AG, Espie GS, Canvin DT. Miller AG, et al. Plant Physiol. 1988 Mar;86(3):677-83. doi: 10.1104/pp.86.3.677. Plant Physiol. 1988. PMID: 16665969 Free PMC article.
• Carbon dioxide transport and carbonic anhydrase in blood and muscle.
  Geers C, Gros G. Geers C, et al. Physiol Rev. 2000 Apr;80(2):681-715. doi: 10.1152/physrev.2000.80.2.681. Physiol Rev. 2000. PMID: 10747205 Review.

Cited by

• Identification and characterization of a carboxysomal γ-carbonic anhydrase from the cyanobacterium Nostoc sp. PCC 7120.
  de Araujo C, Arefeen D, Tadesse Y, Long BM, Price GD, Rowlett RS, Kimber MS, Espie GS. de Araujo C, et al. Photosynth Res. 2014 Sep;121(2-3):135-50. doi: 10.1007/s11120-014-0018-4. Epub 2014 Jun 8. Photosynth Res. 2014. PMID: 24907906
• Association between carbonyl sulfide uptake and (18)Δ during gas exchange in C(3) and C(4) leaves.
  Stimler K, Berry JA, Montzka SA, Yakir D. Stimler K, et al. Plant Physiol. 2011 Sep;157(1):509-17. doi: 10.1104/pp.111.176578. Epub 2011 Jun 29. Plant Physiol. 2011. PMID: 21715674 Free PMC article.
• Carbon Oxysulfide Inhibition of the CO(2)-Concentrating Process of Unicellular Green Algae.
  Goyal A, Shiraiwa Y, Tolbert NE. Goyal A, et al. Plant Physiol. 1992 Feb;98(2):578-83. doi: 10.1104/pp.98.2.578. Plant Physiol. 1992. PMID: 16668680 Free PMC article.
• High Affinity Transport of CO(2) in the Cyanobacterium Synechococcus UTEX 625.
  Espie GS, Miller AG, Canvin DT. Espie GS, et al. Plant Physiol. 1991 Nov;97(3):943-53. doi: 10.1104/pp.97.3.943. Plant Physiol. 1991. PMID: 16668535 Free PMC article.
• Carbon Oxysulfide Is an Inhibitor of Both CO(2) and HCO(3) Uptake in the Cyanobacterium Synechococcus PCC7942.
  Badger MR, Price GD. Badger MR, et al. Plant Physiol. 1990 Sep;94(1):35-9. doi: 10.1104/pp.94.1.35. Plant Physiol. 1990. PMID: 16667708 Free PMC article.

References

1. 1. Plant Physiol. 1988 Jul;87(3):551-4 - PubMed
2. 1. Adv Enzymol Relat Areas Mol Biol. 1978;47:149-274 - PubMed
3. 1. Plant Physiol. 1984 Nov;76(3):599-602 - PubMed
4. 1. Biochem Biophys Res Commun. 1979 Oct 12;90(3):993-9 - PubMed
5. 1. J Biol Chem. 1988 Jul 5;263(19):9199-205 - PubMed

LinkOut - more resources

• Full Text Sources

  • PubMed Central
  • Silverchair Information Systems