Clonal analysis of hematopoietic stem-cell differentiation in vivo - PubMed (original) (raw)

Clonal analysis of hematopoietic stem-cell differentiation in vivo

L G Smith et al. Proc Natl Acad Sci U S A. 1991.

Abstract

Previous work has shown that the 0.02-0.05% of adult mouse bone marrow cells that bear the cell surface phenotype Thy-1loLin-Sca-1+ are enriched 1000- to 2000-fold for hematopoietic stem-cell activity in a variety of assays. When 50-100 cells of this phenotype are injected into an irradiated animal, they can permanently repopulate the entire hematopoietic system. In the present study, limiting-dilution and single-cell experiments were used to address the question of how individual Thy-1loLin-Sca-1+ stem cells contribute to repopulation of the hematopoietic system following irradiation. We calculated that 1 of 13 Thy-1loLin-Sca-1+ cells formed a clone comprising greater than 1% of peripheral white blood cells 3-7 weeks after injection. The majority of these clones included both lymphoid and myeloid lineages. Approximately one-third of the clones continued to produce new blood cells for 9 weeks or more, but the remainder disappeared earlier, including many that were multilineage. Thus, while the majority of Thy-1loLin-Sca-1+ bone marrow cells whose progeny are detected in the in vivo repopulation assay are pluripotential, only a subset undergo long-term self-renewal in vivo. Repopulation appears to be oligoclonal when limiting numbers of Thy-1loLin-Sca-1+ cells are injected. However, the number of clones contributing to hematopoiesis increases in proportion to the number of Thy-1loLin-Sca-1+ cells injected, bringing into question the notion that steady-state hematopoiesis in normal individuals is oligoclonal.

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References

    1. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4634-8 - PubMed
    1. J Exp Med. 1968 Mar 1;127(3):455-64 - PubMed
    1. Cell. 1986 Feb 28;44(4):653-62 - PubMed
    1. Cell. 1986 Jun 20;45(6):917-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8798-802 - PubMed

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