Direct exosome stimulation of peripheral human T cells detected by ELISPOT - PubMed (original) (raw)
. 2006 Jul;36(7):1772-81.
doi: 10.1002/eji.200535615.
Affiliations
- PMID: 16761310
- DOI: 10.1002/eji.200535615
Free article
Direct exosome stimulation of peripheral human T cells detected by ELISPOT
Charlotte Admyre et al. Eur J Immunol. 2006 Jul.
Free article
Abstract
Exosomes from APC are nano-vesicles that can induce antigen-specific T cell responses and are presently explored as therapeutic tools in different clinical settings. Investigations of the capacity of exosomes to stimulate T cells in vitro have mostly been performed on T cell hybridomas, clones or lines. Whether exosomes can stimulate T cells directly or need the presence of dendritic cells (DC) is debated. We could detect exosome-induced antigen-specific CD8(+) T cell responses in peripheral blood from humans. Exosomes from monocyte-derived DC (MDDC) were loaded with a mix of 23 immunogenic peptides from EBV, CMV and influenza virus, and added to autologous peripheral CD8(+) T cells. IFN-gamma-producing cells were detected by enzyme-linked immunospot assay (ELISPOT). MDDC-exosomes induced IFN-gamma production in CD8(+) T cells without addition of DC. The response was exosome dose dependent, and dependent on exosomal MHC class I. Furthermore, we detected an enhanced T cell stimulatory capacity by exosomes from lipopolysaccharide-matured MDDC compared to exosomes from immature MDDC. Exosomes could also induce TNF-alpha production. These results show, for the first time, that exosomes can directly stimulate human peripheral CD8(+) T cells in an antigen-specific manner and that ELISPOT is a suitable method for detecting exosome-induced peripheral T cell responses. This system may provide a useful tool when developing exosomes as therapeutic agents.
Similar articles
- The immunogenicity of dendritic cell-derived exosomes.
Quah BJ, O'Neill HC. Quah BJ, et al. Blood Cells Mol Dis. 2005 Sep-Oct;35(2):94-110. doi: 10.1016/j.bcmd.2005.05.002. Blood Cells Mol Dis. 2005. PMID: 15975838 - Restoration in vitro of impaired T-cell responses in patients with chronic hepatitis B by autologous dendritic cells loaded with hepatitis B virus proteins (R2).
Duan XZ, He HX, Zhuang H. Duan XZ, et al. J Gastroenterol Hepatol. 2006 Jun;21(6):970-6. doi: 10.1111/j.1440-1746.2006.04262.x. J Gastroenterol Hepatol. 2006. PMID: 16724980 - T84-intestinal epithelial exosomes bear MHC class II/peptide complexes potentiating antigen presentation by dendritic cells.
Mallegol J, Van Niel G, Lebreton C, Lepelletier Y, Candalh C, Dugave C, Heath JK, Raposo G, Cerf-Bensussan N, Heyman M. Mallegol J, et al. Gastroenterology. 2007 May;132(5):1866-76. doi: 10.1053/j.gastro.2007.02.043. Epub 2007 Feb 22. Gastroenterology. 2007. PMID: 17484880 - Exosomes - nanovesicles with possible roles in allergic inflammation.
Admyre C, Telemo E, Almqvist N, Lötvall J, Lahesmaa R, Scheynius A, Gabrielsson S. Admyre C, et al. Allergy. 2008 Apr;63(4):404-8. doi: 10.1111/j.1398-9995.2007.01600.x. Allergy. 2008. PMID: 18315728 Review. - Direct stimulation of naïve T cells by antigen-presenting cell vesicles.
Sprent J. Sprent J. Blood Cells Mol Dis. 2005 Jul-Aug;35(1):17-20. doi: 10.1016/j.bcmd.2005.04.004. Blood Cells Mol Dis. 2005. PMID: 15932799 Review.
Cited by
- STING-activating dendritic cell-targeted nanovaccines that evoke potent antigen cross-presentation for cancer immunotherapy.
Nguyen NT, Le XT, Lee WT, Lim YT, Oh KT, Lee ES, Choi HG, Youn YS. Nguyen NT, et al. Bioact Mater. 2024 Sep 6;42:345-365. doi: 10.1016/j.bioactmat.2024.09.002. eCollection 2024 Dec. Bioact Mater. 2024. PMID: 39290338 Free PMC article. - Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response.
Menay F, Cocozza F, Gravisaco MJ, Elisei A, Re JI, Ferella A, Waldner C, Mongini C. Menay F, et al. Front Immunol. 2024 Aug 8;15:1440667. doi: 10.3389/fimmu.2024.1440667. eCollection 2024. Front Immunol. 2024. PMID: 39176090 Free PMC article. - The biology and function of extracellular vesicles in immune response and immunity.
Kalluri R. Kalluri R. Immunity. 2024 Aug 13;57(8):1752-1768. doi: 10.1016/j.immuni.2024.07.009. Immunity. 2024. PMID: 39142276 Review. - Cell-cell communication: new insights and clinical implications.
Su J, Song Y, Zhu Z, Huang X, Fan J, Qiao J, Mao F. Su J, et al. Signal Transduct Target Ther. 2024 Aug 7;9(1):196. doi: 10.1038/s41392-024-01888-z. Signal Transduct Target Ther. 2024. PMID: 39107318 Free PMC article. Review. - Extracellular vesicles at the crossroad between cancer progression and immunotherapy: focus on dendritic cells.
Schioppa T, Gaudenzi C, Zucchi G, Piserà A, Vahidi Y, Tiberio L, Sozzani S, Del Prete A, Bosisio D, Salvi V. Schioppa T, et al. J Transl Med. 2024 Jul 29;22(1):691. doi: 10.1186/s12967-024-05457-4. J Transl Med. 2024. PMID: 39075551 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials