The formation of extracellular matrix during chondrogenic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I - PubMed (original) (raw)
. 2006 Oct;24(10):2252-61.
doi: 10.1634/stemcells.2005-0508. Epub 2006 Jun 15.
Affiliations
- PMID: 16778156
- DOI: 10.1634/stemcells.2005-0508
The formation of extracellular matrix during chondrogenic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I
Christian Prante et al. Stem Cells. 2006 Oct.
Abstract
In vitro differentiation of mesenchymal stem cells (MSCs) into chondrogenic cells and their transplantation is promising as a technique for the treatment of cartilaginous defects. But the regulation of extracellular matrix (ECM) formation remains elusive. Therefore, the objective of this study was to analyze the regulation of proteoglycan (PG) biosynthesis during the chondrogenic differentiation of MSCs. In different stages of chondrogenic differentiation, we analyzed mRNA and protein expression of key enzymes and PG core proteins involved in ECM development. For xylosyltransferase I (XT-I), we found maximum mRNA levels 48 hours after chondrogenic induction with a 5.04 +/- 0.58 (mean +/- SD)-fold increase. This result correlates with significantly elevated levels of enzymatic XT-I activity (0.49 +/- 0.03 muU/1 x 10(6) cells) at this time point. Immunohistochemical staining of XT-I revealed a predominant upregulation in early chondrogenic stages. The highly homologous protein XT-II showed 4.7-fold (SD 0.6) increased mRNA levels on day 7. To determine the differential expression of heparan sulfate (HS), chondroitin sulfate (CS), and dermatan sulfate (DS) chains, we analyzed the mRNA expression of EXTL2 (alpha-4-N-acetylhexosaminyltransferase), GalNAcT (beta-1,4-N-acetylgalactosaminyltransferase), and GlcAC5E (glucuronyl C5 epimerase). All key enzymes showed a similar regulation with temporarily downregulated mRNA levels (up to -87-fold) after chondrogenic induction. In accordance to previous studies, we observed a similar increase in the expression of PG core proteins. In conclusion, we could show that key enzymes for CS, DS, and HS synthesis, especially XT-I, are useful markers for the developmental stages of chondrogenic differentiation.
Similar articles
- Increased levels of xylosyltransferase I correlate with the mineralization of the extracellular matrix during osteogenic differentiation of mesenchymal stem cells.
Müller B, Prante C, Gastens M, Kuhn J, Kleesiek K, Götting C. Müller B, et al. Matrix Biol. 2008 Mar;27(2):139-49. doi: 10.1016/j.matbio.2007.09.005. Epub 2007 Oct 9. Matrix Biol. 2008. PMID: 17980567 - In vitro stage-specific chondrogenesis of mesenchymal stem cells committed to chondrocytes.
Chen WH, Lai MT, Wu AT, Wu CC, Gelovani JG, Lin CT, Hung SC, Chiu WT, Deng WP. Chen WH, et al. Arthritis Rheum. 2009 Feb;60(2):450-9. doi: 10.1002/art.24265. Arthritis Rheum. 2009. PMID: 19180515 - Cartilaginous ECM component-modification of the micro-bead culture system for chondrogenic differentiation of mesenchymal stem cells.
Wu YN, Yang Z, Hui JH, Ouyang HW, Lee EH. Wu YN, et al. Biomaterials. 2007 Oct;28(28):4056-67. doi: 10.1016/j.biomaterials.2007.05.039. Epub 2007 Jun 27. Biomaterials. 2007. PMID: 17590431 - The use of mesenchymal stem cells for chondrogenesis.
Pelttari K, Steck E, Richter W. Pelttari K, et al. Injury. 2008 Apr;39 Suppl 1:S58-65. doi: 10.1016/j.injury.2008.01.038. Injury. 2008. PMID: 18313473 Review. - Directing stem cell differentiation into the chondrogenic lineage in vitro.
Heng BC, Cao T, Lee EH. Heng BC, et al. Stem Cells. 2004;22(7):1152-67. doi: 10.1634/stemcells.2004-0062. Stem Cells. 2004. PMID: 15579636 Review.
Cited by
- Proteoglycans and Glycosaminoglycans in Stem Cell Homeostasis and Bone Tissue Regeneration.
Chen J, Sun T, You Y, Wu B, Wang X, Wu J. Chen J, et al. Front Cell Dev Biol. 2021 Nov 30;9:760532. doi: 10.3389/fcell.2021.760532. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34917612 Free PMC article. Review. - Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.
Lindborg JA, Tran NM, Chenette DM, DeLuca K, Foli Y, Kannan R, Sekine Y, Wang X, Wollan M, Kim IJ, Sanes JR, Strittmatter SM. Lindborg JA, et al. Cell Rep. 2021 Mar 2;34(9):108777. doi: 10.1016/j.celrep.2021.108777. Cell Rep. 2021. PMID: 33657370 Free PMC article. - Expression of xylosyltransferases I and II and their role in the pathogenesis of arthrofibrosis.
Bernstein A, Reichert SNA, Südkamp NP, Hernandez SL, Nerlich AG, Kühle J, Mayr HO. Bernstein A, et al. J Orthop Surg Res. 2020 Jan 23;15(1):27. doi: 10.1186/s13018-020-1544-8. J Orthop Surg Res. 2020. PMID: 31973761 Free PMC article. - Proinflammatory Effects of IL-1β Combined with IL-17A Promoted Cartilage Degradation and Suppressed Genes Associated with Cartilage Matrix Synthesis In Vitro.
Kongdang P, Chokchaitaweesuk C, Tangyuenyong S, Ongchai S. Kongdang P, et al. Molecules. 2019 Oct 13;24(20):3682. doi: 10.3390/molecules24203682. Molecules. 2019. PMID: 31614911 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous