Brief bursts of parallel fiber activity trigger calcium signals in bergmann glia - PubMed (original) (raw)

PF-evoked calcium transients are not triggered by activation of α1-adrenergic receptors, nitric oxide release, CB1 receptors, or AMPA, NMDA, or GABA receptors. Data shown here and in the following figures, except for Figure 6, were obtained in transverse slices. A–D, Graphs on the left plot normalized peak fluorescence transients in BG processes evoked by PF stimulation (10 stimuli, 50 Hz) every 9 min, during bath application of the α1-adrenergic receptor antagonist prazosin (A) (100 μ

m

), the nitric oxide synthase blocker L-NAME (B) (100 μ

m

), the CB1 receptor antagonist AM251 (C) (2 μ

m

), and NBQX (2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[_f_]quinoxaline) (D) (5 μ

m

), CPP 3-[(R)-2-carboxypiperazin-4-yl]-propyl-1-phosphonic acid (5 μ

m

), bicuculline (20 μ

m

), and CGP 55845A (2 μ

m

). Traces (right) show responses from same experiments under control conditions (black) and after bath application of antagonists (gray). E, Summary of effects of antagonists on the amplitude of fluorescence transients (n = 5 cells for each antagonist). Bic, Bicuculline; CGP, CGP 55845A.