Immunofluorescent characterization of retinal ganglion cell neurites cultured on substrates coated with antibodies against Thy-1 - PubMed (original) (raw)
Immunofluorescent characterization of retinal ganglion cell neurites cultured on substrates coated with antibodies against Thy-1
D Leifer et al. Exp Neurol. 1991 Sep.
Abstract
Neurite outgrowth from rat retinal ganglion cells is enhanced if they are cultured on a substrate coated with antibodies against Thy-1. We show that the ganglion cell neurites that grow on antibodies against Thy-1 display immunoreactivity for microtubule-associated protein 2 and tau, cytoskeletal proteins normally localized to the somatodendritic and axonal compartments, respectively. This result suggests that these neurites are incompletely differentiated and have axonal and dendritic features. We also show that the antibodies against Thy-1 used to coat the substrate bind specifically to ganglion cell membranes within 1 day of culture.
Similar articles
- Selectivity of Thy-1 monoclonal antibodies in enhancing neurite outgrowth.
Lipton SA, Leifer D, Barnstable CJ. Lipton SA, et al. Neurosci Lett. 1992 Mar 16;137(1):75-7. doi: 10.1016/0304-3940(92)90302-n. Neurosci Lett. 1992. PMID: 1352632 - Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture.
Leifer D, Lipton SA, Barnstable CJ, Masland RH. Leifer D, et al. Science. 1984 Apr 20;224(4646):303-6. doi: 10.1126/science.6143400. Science. 1984. PMID: 6143400 - Thy-1 is critical for normal retinal development.
Simon PD, McConnell J, Zurakowski D, Vorwerk CK, Naskar R, Grosskreutz CL, Dreyer EB. Simon PD, et al. Brain Res Dev Brain Res. 1999 Nov 18;117(2):219-23. doi: 10.1016/s0165-3806(99)00123-6. Brain Res Dev Brain Res. 1999. PMID: 10567740 - NMDA sensitivity is neurite enhanced.
Heng JE, Moscaritolo K, Dreyer EB. Heng JE, et al. Neuroreport. 1995 Oct 2;6(14):1890-2. doi: 10.1097/00001756-199510020-00016. Neuroreport. 1995. PMID: 8547591 - Growth cone motility.
Cypher C, Letourneau PC. Cypher C, et al. Curr Opin Cell Biol. 1992 Feb;4(1):4-7. doi: 10.1016/0955-0674(92)90051-d. Curr Opin Cell Biol. 1992. PMID: 1558753 Review.
Cited by
- An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
Banerjee SA, Hadjiargyrou M, Patterson PH. Banerjee SA, et al. J Neurosci. 1997 Apr 15;17(8):2756-65. doi: 10.1523/JNEUROSCI.17-08-02756.1997. J Neurosci. 1997. PMID: 9092597 Free PMC article. - An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth.
Dreyer EB, Leifer D, Heng JE, McConnell JE, Gorla M, Levin LA, Barnstable CJ, Lipton SA. Dreyer EB, et al. Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11195-9. doi: 10.1073/pnas.92.24.11195. Proc Natl Acad Sci U S A. 1995. PMID: 7479964 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Miscellaneous