Synovial fluid leukocyte apoptosis is inhibited in patients with very early rheumatoid arthritis - PubMed (original) (raw)
Dagmar Scheel-Toellner, Chi-Yeung Lee, Darrell Pilling, S John Curnow, Francesco Falciani, Victor Trevino, Kanta Kumar, Lakhvir K Assi, Janet M Lord, Caroline Gordon, Christopher D Buckley, Mike Salmon
Affiliations
- PMID: 16859518
- PMCID: PMC1779404
- DOI: 10.1186/ar2009
Synovial fluid leukocyte apoptosis is inhibited in patients with very early rheumatoid arthritis
Karim Raza et al. Arthritis Res Ther. 2006.
Abstract
Synovial leukocyte apoptosis is inhibited in established rheumatoid arthritis (RA). In contrast, high levels of leukocyte apoptosis are seen in self-limiting crystal arthritis. The phase in the development of RA at which the inhibition of leukocyte apoptosis is first apparent, and the relationship between leukocyte apoptosis in early RA and other early arthritides, has not been defined. We measured synovial fluid leukocyte apoptosis in very early arthritis and related this to clinical outcome. Synovial fluid was obtained at presentation from 81 patients with synovitis of < or = 3 months duration. The percentages of apoptotic neutrophils and lymphocytes were assessed on cytospin preparations. Patients were assigned to diagnostic groups after 18 months follow-up. The relationship between leukocyte apoptosis and patient outcome was assessed. Patients with early RA had significantly lower levels of neutrophil apoptosis than patients who developed non-RA persistent arthritis and those with a resolving disease course. Similarly, lymphocyte apoptosis was absent in patients with early RA whereas it was seen in patients with other early arthritides. The inhibition of synovial fluid leukocyte apoptosis in the earliest clinically apparent phase of RA distinguishes this from other early arthritides. The mechanisms for this inhibition may relate to the high levels of anti-apoptotic cytokines found in the early rheumatoid joint (e.g. IL-2, IL-4, IL-15 GMCSF, GCSF). It is likely that this process contributes to an accumulation of leukocytes in the early rheumatoid lesion and is involved in the development of the microenvironment required for persistent RA.
Figures
Figure 1
Apoptotic lymphocytes and neutrophils in synovial fluid cytospin preparations from patients with very early inflammatory arthritis. (a) An apoptotic lymphocyte (dashed arrow). (b) An apoptotic lymphocyte (dashed arrow) and apoptotic neutrophil (solid arrow). (c) Four apoptotic neutrophils (solid arrows).
Figure 2
Synovial fluid neutrophil and lymphocyte apoptosis in patients with very early inflammatory arthritis. (a) Synovial fluid neutrophil apoptosis at clinical presentation in patients with very early inflammatory arthritis divided according to outcome. (b) Synovial fluid lymphocyte apoptosis at clinical presentation in patients with very early inflammatory arthritis divided according to outcome. (c) Maximum synovial fluid neutrophil apoptosis observed in each patient with very early inflammatory arthritis; patients divided according to outcome. (d) Maximum synovial fluid lymphocyte apoptosis in each patient with very early inflammatory arthritis; patients divided according to outcome. (e) Synovial fluid neutrophil apoptosis over time in all samples obtained from patients with very early inflammatory arthritis that eventually persisted divided according to outcome (open circles = non-RA persistent synovitis; closed circles = RA). (f) Synovial fluid lymphocyte apoptosis over time in all samples obtained from patients with very early inflammatory arthritis that eventually persisted divided according to outcome (open circles = non-RA persistent synovitis; closed circles = RA). Comparisons were made using the Mann-Whitney test. RA, rheumatoid arthritis.
Similar articles
- Early rheumatoid arthritis is characterized by a distinct and transient synovial fluid cytokine profile of T cell and stromal cell origin.
Raza K, Falciani F, Curnow SJ, Ross EJ, Lee CY, Akbar AN, Lord JM, Gordon C, Buckley CD, Salmon M. Raza K, et al. Arthritis Res Ther. 2005;7(4):R784-95. doi: 10.1186/ar1733. Epub 2005 Apr 7. Arthritis Res Ther. 2005. PMID: 15987480 Free PMC article. - Synovial fluid from patients with rheumatoid arthritis inhibits neutrophil apoptosis: role of adenosine and proinflammatory cytokines.
Ottonello L, Cutolo M, Frumento G, Arduino N, Bertolotto M, Mancini M, Sottofattori E, Dallegri F. Ottonello L, et al. Rheumatology (Oxford). 2002 Nov;41(11):1249-60. doi: 10.1093/rheumatology/41.11.1249. Rheumatology (Oxford). 2002. PMID: 12421997 - Synovial lymphoid neogenetic factors in Behçet's synovitis: do they play a role in self-limiting and subacute course of arthritis?
Pay S, Musabak U, Simsek I, Erdem H, Pekel A, Sengul A, Dinc A. Pay S, et al. Clin Exp Rheumatol. 2007 Jul-Aug;25(4 Suppl 45):S21-6. Clin Exp Rheumatol. 2007. PMID: 17949547 - The histopathology of early synovitis.
Hitchon CA, el-Gabalawy HS. Hitchon CA, et al. Clin Exp Rheumatol. 2003 Sep-Oct;21(5 Suppl 31):S28-36. Clin Exp Rheumatol. 2003. PMID: 14969047 Review. - [Cytokines in rheumatoid arthritis].
Robak T, Gladalska A. Robak T, et al. Postepy Hig Med Dosw. 1997;51(6):621-36. Postepy Hig Med Dosw. 1997. PMID: 9481895 Review. Polish.
Cited by
- Association of Serum IL-17 and IL-23 Cytokines With Disease Activity and Various Parameters of Rheumatoid Arthritis in Indian Patients.
Meher J, Patel S, Nanda R, Siddiqui MS. Meher J, et al. Cureus. 2023 Nov 29;15(11):e49654. doi: 10.7759/cureus.49654. eCollection 2023 Nov. Cureus. 2023. PMID: 38161845 Free PMC article. - Seasonal variation and disease distribution of cytophagocytic mononuclear cells in synovial fluid: A medical record study.
Oliviero F, Galozzi P, Padoan A, Lorenzin M, Scanu A, Ramonda R. Oliviero F, et al. Exp Biol Med (Maywood). 2023 May;248(10):839-842. doi: 10.1177/15353702231162078. Epub 2023 Apr 24. Exp Biol Med (Maywood). 2023. PMID: 37092758 Free PMC article. - Inflammatory-associated apoptotic markers: are they the culprit to rheumatoid arthritis pain?
Khir NAM, Noh ASM, Long I, Ismail NI, Siran R, Ismail CAN. Khir NAM, et al. Mol Biol Rep. 2022 Oct;49(10):10077-10090. doi: 10.1007/s11033-022-07591-y. Epub 2022 Jun 14. Mol Biol Rep. 2022. PMID: 35699858 Review. - Comprehensive transcriptomic analysis shows disturbed calcium homeostasis and deregulation of T lymphocyte apoptosis in inclusion body myositis.
Johari M, Vihola A, Palmio J, Jokela M, Jonson PH, Sarparanta J, Huovinen S, Savarese M, Hackman P, Udd B. Johari M, et al. J Neurol. 2022 Aug;269(8):4161-4173. doi: 10.1007/s00415-022-11029-7. Epub 2022 Mar 2. J Neurol. 2022. PMID: 35237874 Free PMC article. - Anti-Carbamylated LL37 Antibodies Promote Pathogenic Bone Resorption in Rheumatoid Arthritis.
O'Neil LJ, Oliveira CB, Sandoval-Heglund D, Barrera-Vargas A, Merayo-Chalico J, Aguirre-Aguilar E, Kaplan MJ, Carmona-Rivera C. O'Neil LJ, et al. Front Immunol. 2021 Sep 14;12:715997. doi: 10.3389/fimmu.2021.715997. eCollection 2021. Front Immunol. 2021. PMID: 34594331 Free PMC article.
References
- Pilling D, Akbar AN, Girdlestone J, Orteu CH, Borthwick NJ, Amft N, Scheel-Toellner D, Buckley CD, Salmon M. Interferon-beta mediates stromal cell rescue of T cells from apoptosis. Eur J Immunol. 1999;29:1041–1050. doi: 10.1002/(SICI)1521-4141(199903)29:03<1041::AID-IMMU1041>3.0.CO;2-#. - DOI - PubMed
- Scheel-Toellner D, Wang K, Henriquez NV, Webb PR, Craddock R, Pilling D, Akbar AN, Salmon M, Lord JM. Cytokine-mediated inhibition of apoptosis in non-transformed T cells and neutrophils can bedissociated from protein kinase B activation. Eur J Immunol. 2002;32:486–493. doi: 10.1002/1521-4141(200202)32:2<486::AID-IMMU486>3.0.CO;2-U. - DOI - PubMed
- Ottonello L, Cutolo M, Frumento G, Arduino N, Bertolotto M, Mancini M, Sottofattori E, Dallegri F. Synovial fluid from patients with rheumatoid arthritis inhibits neutrophil apoptosis: role of adenosine and proinflammatory cytokines. Rheumatology (Oxford) 2002;41:1249–1260. doi: 10.1093/rheumatology/41.11.1249. - DOI - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical