Involvement of calcium-sensing receptor in ischemia/reperfusion-induced apoptosis in rat cardiomyocytes - PubMed (original) (raw)
. 2006 Sep 8;347(4):872-81.
doi: 10.1016/j.bbrc.2006.06.176. Epub 2006 Jul 10.
Song-bin Fu, Fang-hao Lu, Bo Wu, Dong-mei Gong, Zhen-wei Pan, Yan-jie Lv, Ya-jun Zhao, Quan-Feng Li, Rui Wang, Bao-feng Yang, Chang-qing Xu
Affiliations
- PMID: 16859639
- DOI: 10.1016/j.bbrc.2006.06.176
Involvement of calcium-sensing receptor in ischemia/reperfusion-induced apoptosis in rat cardiomyocytes
Wei-hua Zhang et al. Biochem Biophys Res Commun. 2006.
Abstract
The calcium-sensing receptor (CaR) is a seven-transmembrane G-protein coupled receptor, which activates intracellular effectors, for example, it causes inositol phosphate (IP) accumulation to increase the release of intracellular calcium. Although intracellular calcium overload has been implicated in the cardiac ischemia/reperfusion (I/R)-induced apoptosis, the role of CaR in the induction of apoptosis has not been fully understood. This study tested the hypothesis that CaR is involved in I/R cardiomyocyte apoptosis by increasing [Ca2+]i. The isolated rat hearts were subjected to 40-min ischemia followed by 2 h of reperfusion, meanwhile GdCl3 was added to reperfusion solution. The expression of CaR increased at the exposure to GdCl3 during I/R. By laser confocal microscopy, it was observed that the intracellular calcium was significantly increased and exhibited a Deltapsim, as monitored by 5,5',6,6'-tetrachloro-1,1',3,3'- tetraethylbenzimidazolcarbocyanine iodide (JC-1) during reperfusion with GdCl3. Furthermore, the number of apoptotic cells was significantly increased as shown by TUNEL assay. Typical apoptotic cells were observed with transmission electron microscopy in I/R with GdCl3 but not in the control group. The expression of cytosolic cytochrome c and activated caspase-9 and caspase-3 was significantly increased whereas the expression of mitochondrial cytochrome c significantly decreased in I/R with GdCl3 in comparison to the control. In conclusion, these results suggest that CaR is involved in the induction of cardiomyocyte apoptosis during ischemia/reperfusion through activation of cytochrome c-caspase-3 signaling pathway.
Similar articles
- [The role of calcium-sensing receptor on ischemia/reperfusion-induced rat cardiomyocyte apoptosis].
Zhang WC, Zhang WH, Wu B, Zhao YJ, Li QF, Xu CQ. Zhang WC, et al. Zhonghua Xin Xue Guan Bing Za Zhi. 2007 Aug;35(8):740-4. Zhonghua Xin Xue Guan Bing Za Zhi. 2007. PMID: 17963636 Chinese. - Calcium-sensing receptors induce apoptosis in rat cardiomyocytes via the endo(sarco)plasmic reticulum pathway during hypoxia/reoxygenation.
Lu F, Tian Z, Zhang W, Zhao Y, Bai S, Ren H, Chen H, Yu X, Wang J, Wang L, Li H, Pan Z, Tian Y, Yang B, Wang R, Xu C. Lu F, et al. Basic Clin Pharmacol Toxicol. 2010 May;106(5):396-405. doi: 10.1111/j.1742-7843.2009.00502.x. Epub 2009 Dec 22. Basic Clin Pharmacol Toxicol. 2010. PMID: 20030631 - Effect of dopamine receptor 1 on apoptosis of cultured neonatal rat cardiomyocytes in simulated ischaemia/reperfusion.
Li HZ, Han LP, Jiang CM, Li H, Zhao YJ, Gao J, Lin Y, Ma SX, Tian Y, Yang BF, Xu CQ. Li HZ, et al. Basic Clin Pharmacol Toxicol. 2008 Mar;102(3):329-36. doi: 10.1111/j.1742-7843.2007.00177.x. Basic Clin Pharmacol Toxicol. 2008. PMID: 18294176 - Oxidative stress-elicited myocardial apoptosis during reperfusion.
Zhao ZQ. Zhao ZQ. Curr Opin Pharmacol. 2004 Apr;4(2):159-65. doi: 10.1016/j.coph.2003.10.010. Curr Opin Pharmacol. 2004. PMID: 15063360 Review. - Functional and clinical repercussions of myocyte apoptosis in the multifaceted damage by ischemia/reperfusion injury: old and new concepts after 10 years of contributions.
Scarabelli TM, Gottlieb RA. Scarabelli TM, et al. Cell Death Differ. 2004 Dec;11 Suppl 2:S144-52. doi: 10.1038/sj.cdd.4401544. Cell Death Differ. 2004. PMID: 15608693 Review.
Cited by
- VEGF121 Mediates Post-Hypoxia Cardioprotective Effects Via CaSR and Mitochondria-Dependent Protease Pathway.
Zhang Y, Yin WH, Yang F, An YQ, Zhou W, Yu H, Xie H, Zhang YL, Zhu Y, Shen XC, Tian R. Zhang Y, et al. Arq Bras Cardiol. 2021 Sep;117(3):476-483. doi: 10.36660/abc.20190902. Arq Bras Cardiol. 2021. PMID: 34550233 Free PMC article. English, Portuguese. - Calcium sensing receptor involving in therapy of embryonic stem cell transplantation alleviates acute myocardial infarction by inhibiting apoptosis and oxidative stress in rats.
Yuan H, Yang G, Li S, Li L, Wei T, Song G, Luan H, Meng J, Wang Q, Yu Y, Sun J. Yuan H, et al. Iran J Basic Med Sci. 2020 Oct;23(10):1353-1359. doi: 10.22038/ijbms.2020.47436.10916. Iran J Basic Med Sci. 2020. PMID: 33149870 Free PMC article. - Calhex231 ameliorates myocardial fibrosis post myocardial infarction in rats through the autophagy-NLRP3 inflammasome pathway in macrophages.
Liu W, Sun J, Guo Y, Liu N, Ding X, Zhang X, Chi J, Kang N, Liu Y, Yin X. Liu W, et al. J Cell Mol Med. 2020 Nov;24(22):13440-13453. doi: 10.1111/jcmm.15969. Epub 2020 Oct 12. J Cell Mol Med. 2020. PMID: 33043596 Free PMC article. - Activation of calcium‑sensing receptor‑mediated autophagy in high glucose‑induced cardiac fibrosis in vitro.
Yuan H, Xu J, Zhu Y, Li L, Wang Q, Yu Y, Zhou B, Liu Y, Xu X, Wang Z. Yuan H, et al. Mol Med Rep. 2020 Sep;22(3):2021-2031. doi: 10.3892/mmr.2020.11277. Epub 2020 Jun 26. Mol Med Rep. 2020. PMID: 32705187 Free PMC article. - Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.
Wang Y, Chen J, Li S, Zhang X, Guo Z, Hu J, Shao X, Song N, Zhao Y, Li H, Yang G, Xu C, Wei C. Wang Y, et al. Redox Biol. 2020 May;32:101514. doi: 10.1016/j.redox.2020.101514. Epub 2020 Mar 21. Redox Biol. 2020. PMID: 32234613 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous