Crystal structure of a secretory signalling glycoprotein from sheep at 2.0A resolution - PubMed (original) (raw)
. 2006 Dec;156(3):505-16.
doi: 10.1016/j.jsb.2006.05.008. Epub 2006 Jun 8.
Affiliations
- PMID: 16859926
- DOI: 10.1016/j.jsb.2006.05.008
Crystal structure of a secretory signalling glycoprotein from sheep at 2.0A resolution
Devendra B Srivastava et al. J Struct Biol. 2006 Dec.
Abstract
A 40kDa glycoprotein from dry secretion of sheep is implicated as a signaling factor and is named as SPS-40. This protein is secreted only during the early phase of involution when the drastic tissue remodeling occurs in the mammary gland. SPS-40 was purified from sheep dry secretions and crystallized using hanging drop vapour diffusion method. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions, a=62.7A, b=66.4A, c=107.5A. The protein was also cloned for the determination of its complete amino acid sequence. The three-dimensional structure of SPS-40 was determined by X-ray crystallographic method at 2.0A resolution. The structure revealed the presence of an N-linked glycan chain at Asn39. The protein adopts a conformation with a classical (beta/alpha)(8)-barrel fold of triosephosphate isomerase (TIM) (residues 1-237 and 310-360) with an insertion of a small (alpha+beta) domain (residues 240-307) similar to that observed in chitinases. However, the Leu substitution for Glu in the consensus catalytic sequence in SPS-40 causes a loss of chitinase activity. Furthermore, the sugar-binding groove in SPS-40 is distorted considerably from the standard chitin-binding site in chitinase enzymes and hence the binding of chitin-like oligosaccharides is considerably hampered. Three surface loops, His188-His197, Phe202-Arg212 and Phe244-Pro260 have exceptionally high values of B-factors (average=70.5A(2)), indicating the presence of a less defined region.
Similar articles
- Structure of a bovine secretory signalling glycoprotein (SPC-40) at 2.1 Angstrom resolution.
Kumar J, Ethayathulla AS, Srivastava DB, Sharma S, Singh SB, Srinivasan A, Yadav MP, Singh TP. Kumar J, et al. Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):953-63. doi: 10.1107/S0907444906020427. Epub 2006 Aug 19. Acta Crystallogr D Biol Crystallogr. 2006. PMID: 16929095 - Carbohydrate binding properties and carbohydrate induced conformational switch in sheep secretory glycoprotein (SPS-40): crystal structures of four complexes of SPS-40 with chitin-like oligosaccharides.
Srivastava DB, Ethayathulla AS, Kumar J, Somvanshi RK, Sharma S, Dey S, Singh TP. Srivastava DB, et al. J Struct Biol. 2007 Jun;158(3):255-66. doi: 10.1016/j.jsb.2006.11.002. Epub 2006 Nov 17. J Struct Biol. 2007. PMID: 17188513 - Tryptophan as a three-way switch in regulating the function of the secretory signalling glycoprotein (SPS-40) from mammary glands: structure of SPS-40 complexed with 2-methylpentane-2,4-diol at 1.6 A resolution.
Sharma P, Singh N, Sinha M, Sharma S, Perbandt M, Betzel C, Kaur P, Srinivasan A, Singh TP. Sharma P, et al. Acta Crystallogr D Biol Crystallogr. 2009 Apr;65(Pt 4):375-8. doi: 10.1107/S0907444909002327. Epub 2009 Mar 19. Acta Crystallogr D Biol Crystallogr. 2009. PMID: 19307719 - Structure-function studies on beta 2-glycoprotein I.
de Groot G PG, Bouma B, Lutters BC, Simmelink MJ, Derksen RH, Gros P. de Groot G PG, et al. J Autoimmun. 2000 Sep;15(2):87-9. doi: 10.1006/jaut.2000.0394. J Autoimmun. 2000. PMID: 10968890 Review. - Structure-based enzyme engineering and its impact on in vitro glycorandomization.
Thorson JS, Barton WA, Hoffmeister D, Albermann C, Nikolov DB. Thorson JS, et al. Chembiochem. 2004 Jan 3;5(1):16-25. doi: 10.1002/cbic.200300620. Chembiochem. 2004. PMID: 14695508 Review. No abstract available.
Cited by
- Structure of the buffalo secretory signalling glycoprotein at 2.8 A resolution.
Ethayathulla AS, Srivastava DB, Kumar J, Saravanan K, Bilgrami S, Sharma S, Kaur P, Srinivasan A, Singh TP. Ethayathulla AS, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):258-65. doi: 10.1107/S1744309107010445. Epub 2007 Mar 12. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007. PMID: 17401190 Free PMC article. - Evolution of mammalian chitinase(-like) members of family 18 glycosyl hydrolases.
Bussink AP, Speijer D, Aerts JM, Boot RG. Bussink AP, et al. Genetics. 2007 Oct;177(2):959-70. doi: 10.1534/genetics.107.075846. Epub 2007 Aug 24. Genetics. 2007. PMID: 17720922 Free PMC article. - Structural and functional characterization of buffalo oviduct-specific glycoprotein (OVGP1) expressed during estrous cycle.
Choudhary S, Janjanam J, Kumar S, Kaushik JK, Mohanty AK. Choudhary S, et al. Biosci Rep. 2019 Dec 20;39(12):BSR20191501. doi: 10.1042/BSR20191501. Biosci Rep. 2019. PMID: 31763672 Free PMC article. - Modulation of inhibitory activity of xylanase-α-amylase inhibitor protein (XAIP): binding studies and crystal structure determination of XAIP-II from Scadoxus multiflorus at 1.2 Å resolution.
Kumar S, Singh N, Mishra B, Dube D, Sinha M, Singh SB, Dey S, Kaur P, Sharma S, Singh TP. Kumar S, et al. BMC Struct Biol. 2010 Nov 20;10:41. doi: 10.1186/1472-6807-10-41. BMC Struct Biol. 2010. PMID: 21092126 Free PMC article. - Functional analysis of an unusual porin-like channel that imports chitin for alternative carbon metabolism in Escherichia coli.
Soysa HSM, Schulte A, Suginta W. Soysa HSM, et al. J Biol Chem. 2017 Nov 24;292(47):19328-19337. doi: 10.1074/jbc.M117.812321. Epub 2017 Sep 27. J Biol Chem. 2017. PMID: 28972167 Free PMC article.