Embryonic expression of zebrafish AMPA receptor genes: zygotic gria2alpha expression initiates at the midblastula transition - PubMed (original) (raw)
Comparative Study
. 2006 Sep 19;1110(1):46-54.
doi: 10.1016/j.brainres.2006.06.054. Epub 2006 Aug 2.
Affiliations
- PMID: 16887104
- DOI: 10.1016/j.brainres.2006.06.054
Comparative Study
Embryonic expression of zebrafish AMPA receptor genes: zygotic gria2alpha expression initiates at the midblastula transition
Wei-Hsiang Lin et al. Brain Res. 2006.
Abstract
The AMPA-preferring receptors (AMPARs) mediate rapid excitatory synaptic transmission in the central nervous system of vertebrates. Expression profiles of 8 AMPAR genes were studied by RT-PCR analyses to elucidate the properties of AMPARs during early zebrafish development. Transcripts of all AMPAR genes are detected at the time of fertilization, suggesting maternal transcriptions of zebrafish AMPAR genes. The amounts of gria1 and gria2 transcripts are several-fold higher than that of gria3 and gria4 between 10 and 72 hpf (hour postfertilization). The edited gria2alpha transcript decreases during gastrulation period, suggesting that zygotic expression of gria2alpha begins around the time of midblastula transition. Relative to the amount of beta-actin, the amounts of AMPAR transcripts increase significantly after the completion of neurulation. The amounts of gria2 transcripts exceed the total amounts of the remaining AMPAR transcripts after 36 hpf, suggesting increases in the representation of low Ca2+ permeable AMPARs during neuronal maturation. Many but not all of the known mammalian protein-protein interaction motifs are preserved in the C-terminal domains (CTD) of zebrafish AMPARs. Before 16 hpf, the embryos express predominantly the alternative splice forms encoding longer CTD. Representations of the short CTD splice forms of gria2 and gria4alpha increase after 24 hpf, when neurulation is nearly completed.
Similar articles
- Identifications, classification, and evolution of the vertebrate alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunit genes.
Chen YC, Kung SS, Chen BY, Hung CC, Chen CC, Wang TY, Wu YM, Lin WH, Tzeng CS, Chow WY. Chen YC, et al. J Mol Evol. 2001 Dec;53(6):690-702. doi: 10.1007/s002390010256. J Mol Evol. 2001. PMID: 11677629 - Expression analysis of steroid hormone receptor mRNAs during zebrafish embryogenesis.
Pikulkaew S, De Nadai A, Belvedere P, Colombo L, Dalla Valle L. Pikulkaew S, et al. Gen Comp Endocrinol. 2010 Jan 15;165(2):215-20. doi: 10.1016/j.ygcen.2009.06.024. Epub 2009 Jul 2. Gen Comp Endocrinol. 2010. PMID: 19576895 - Expression of the eight AMPA receptor subunit genes in the developing central nervous system and sensory organs of zebrafish.
Hoppmann V, Wu JJ, Søviknes AM, Helvik JV, Becker TS. Hoppmann V, et al. Dev Dyn. 2008 Mar;237(3):788-99. doi: 10.1002/dvdy.21447. Dev Dyn. 2008. PMID: 18224707 - The knockdown of the maternal estrogen receptor 2a (esr2a) mRNA affects embryo transcript contents and larval development in zebrafish.
Celeghin A, Benato F, Pikulkaew S, Rabbane MG, Colombo L, Dalla Valle L. Celeghin A, et al. Gen Comp Endocrinol. 2011 May 15;172(1):120-9. doi: 10.1016/j.ygcen.2010.12.020. Epub 2011 Jan 1. Gen Comp Endocrinol. 2011. PMID: 21199655 - A tiny helper lightens the maternal load.
Weigel D, Izaurralde E. Weigel D, et al. Cell. 2006 Mar 24;124(6):1117-8. doi: 10.1016/j.cell.2006.03.005. Cell. 2006. PMID: 16564001 Review.
Cited by
- A Critical Review of Zebrafish Neurological Disease Models-1. The Premise: Neuroanatomical, Cellular and Genetic Homology and Experimental Tractability.
Burgess HA, Burton EA. Burgess HA, et al. Oxf Open Neurosci. 2023 Jan 6;2:kvac018. doi: 10.1093/oons/kvac018. eCollection 2023. Oxf Open Neurosci. 2023. PMID: 37649777 Free PMC article. - Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio).
Shah SM, Wahba M, Yu L, Achari G, Habibi HR. Shah SM, et al. Toxics. 2019 Aug 23;7(3):42. doi: 10.3390/toxics7030042. Toxics. 2019. PMID: 31450778 Free PMC article. - Zebrafish Get Connected: Investigating Neurotransmission Targets and Alterations in Chemical Toxicity.
Horzmann KA, Freeman JL. Horzmann KA, et al. Toxics. 2016 Sep;4(3):19. doi: 10.3390/toxics4030019. Epub 2016 Aug 27. Toxics. 2016. PMID: 28730152 Free PMC article. - AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate.
Hirai S, Hotta K, Kubo Y, Nishino A, Okabe S, Okamura Y, Okado H. Hirai S, et al. Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):3939-3944. doi: 10.1073/pnas.1612943114. Epub 2017 Mar 27. Proc Natl Acad Sci U S A. 2017. PMID: 28348228 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous