Manufacturing of recombinant therapeutic proteins in microbial systems - PubMed (original) (raw)

Review

Manufacturing of recombinant therapeutic proteins in microbial systems

Klaus Graumann et al. Biotechnol J. 2006 Feb.

Abstract

Recombinant therapeutic proteins have gained enormous importance for clinical applications. The first recombinant products have been produced in E. coli more than 20 years ago. Although with the advent of antibody-based therapeutics mammalian expression systems have experienced a major boost, microbial expression systems continue to be widely used in industry. Their intrinsic advantages, such as rapid growth, high yields and ease of manipulation, make them the premier choice for expression of non-glycosylated peptides and proteins. Innovative product classes such as antibody fragments or alternative binding molecules will further expand the use of microbial systems. Even more, novel, engineered production hosts and integrated technology platforms hold enormous potential for future applications. This review summarizes current applications and trends for development, production and analytical characterization of recombinant therapeutic proteins in microbial systems.

PubMed Disclaimer

Comment in

• The art of bioprocessing.
  Jungbauer A. Jungbauer A. Biotechnol J. 2006 Feb;1(2):111. doi: 10.1002/biot.200690030. Biotechnol J. 2006. PMID: 16892235 No abstract available.

Similar articles

• Antibodies and genetically engineered related molecules: production and purification.
  Roque AC, Lowe CR, Taipa MA. Roque AC, et al. Biotechnol Prog. 2004 May-Jun;20(3):639-54. doi: 10.1021/bp030070k. Biotechnol Prog. 2004. PMID: 15176864 Review.
• Proteomic profiling of Escherichia coli proteins under high cell density fed-batch cultivation with overexpression of phosphogluconolactonase.
  Wang Y, Wu SL, Hancock WS, Trala R, Kessler M, Taylor AH, Patel PS, Aon JC. Wang Y, et al. Biotechnol Prog. 2005 Sep-Oct;21(5):1401-11. doi: 10.1021/bp050048m. Biotechnol Prog. 2005. PMID: 16209543
• Engineering cell physiology to enhance recombinant protein production in Escherichia coli.
  Chou CP. Chou CP. Appl Microbiol Biotechnol. 2007 Sep;76(3):521-32. doi: 10.1007/s00253-007-1039-0. Epub 2007 Jun 15. Appl Microbiol Biotechnol. 2007. PMID: 17571257 Review.
• Plant cell cultures for the production of recombinant proteins.
  Hellwig S, Drossard J, Twyman RM, Fischer R. Hellwig S, et al. Nat Biotechnol. 2004 Nov;22(11):1415-22. doi: 10.1038/nbt1027. Nat Biotechnol. 2004. PMID: 15529167 Review.
• The rapid discovery of engineered antibodies.
  Wang M, He M. Wang M, et al. IDrugs. 2007 Aug;10(8):562-5. IDrugs. 2007. PMID: 17665332 Review.

Cited by

• Differentiating biosimilarity and comparability in biotherapeutics.
  Azevedo V, Hassett B, Fonseca JE, Atsumi T, Coindreau J, Jacobs I, Mahgoub E, O'Brien J, Singh E, Vicik S, Fitzpatrick B. Azevedo V, et al. Clin Rheumatol. 2016 Dec;35(12):2877-2886. doi: 10.1007/s10067-016-3427-2. Epub 2016 Oct 12. Clin Rheumatol. 2016. PMID: 27734233 Review.
• Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens.
  Popovic M, Prodanovic R, Ostafe R, Schillberg S, Fischer R, Gavrovic-Jankulovic M. Popovic M, et al. Immunol Res. 2015 Mar;61(3):230-9. doi: 10.1007/s12026-014-8614-0. Immunol Res. 2015. PMID: 25537533
• A protocol for recombinant protein quantification by densitometry.
  Alonso Villela SM, Kraïem H, Bouhaouala-Zahar B, Bideaux C, Aceves Lara CA, Fillaudeau L. Alonso Villela SM, et al. Microbiologyopen. 2020 Jun;9(6):1175-1182. doi: 10.1002/mbo3.1027. Epub 2020 Apr 7. Microbiologyopen. 2020. PMID: 32255275 Free PMC article.
• Production of single chain Fab (scFab) fragments in Bacillus megaterium.
  Jordan E, Al-Halabi L, Schirrmann T, Hust M, Dübel S. Jordan E, et al. Microb Cell Fact. 2007 Nov 27;6:38. doi: 10.1186/1475-2859-6-38. Microb Cell Fact. 2007. PMID: 18042285 Free PMC article.
• The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis.
  Baumann K, Dato L, Graf AB, Frascotti G, Dragosits M, Porro D, Mattanovich D, Ferrer P, Branduardi P. Baumann K, et al. BMC Genomics. 2011 May 9;12:218. doi: 10.1186/1471-2164-12-218. BMC Genomics. 2011. PMID: 21554735 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database