Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters - PubMed (original) (raw)

. 2006 Sep 22;348(2):716-21.

doi: 10.1016/j.bbrc.2006.07.136. Epub 2006 Jul 31.

Affiliations

• PMID: 16895723
• DOI: 10.1016/j.bbrc.2006.07.136

Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters

Michael D Allen et al. Biochem Biophys Res Commun. 2006.

Abstract

cAMP-dependent protein kinase (PKA) mediates key cellular processes via compartmentalized activity, and the ability to track its activity in living cells should help increase our understanding of this precise regulation. Here, through systematic testing of new fluorescent proteins, we developed a new FRET-based A-kinase activity reporter (AKAR), AKAR3, with a dynamic range of 31-41%, twice that of predecessors. Visualization of PKA activity at plasma membrane, cytoplasm, nucleus, and mitochondria was achieved. Targeting AKAR3 to outer mitochondrial membrane revealed that basal PKA activity at mitochondria differs from that in the cytoplasm, indicating differential regulation of PKA activity at different subcellular locations.

PubMed Disclaimer

Similar articles

• Analyzing protein kinase dynamics in living cells with FRET reporters.
  Ni Q, Titov DV, Zhang J. Ni Q, et al. Methods. 2006 Nov;40(3):279-86. doi: 10.1016/j.ymeth.2006.06.013. Methods. 2006. PMID: 16908183 Review.
• Small-molecule FRET probes for protein kinase activity monitoring in living cells.
  Vaasa A, Lust M, Terrin A, Uri A, Zaccolo M. Vaasa A, et al. Biochem Biophys Res Commun. 2010 Jul 9;397(4):750-5. doi: 10.1016/j.bbrc.2010.06.026. Epub 2010 Jun 10. Biochem Biophys Res Commun. 2010. PMID: 20541535
• Measuring dynamic changes in cAMP using fluorescence resonance energy transfer.
  Evellin S, Mongillo M, Terrin A, Lissandron V, Zaccolo M. Evellin S, et al. Methods Mol Biol. 2004;284:259-70. doi: 10.1385/1-59259-816-1:259. Methods Mol Biol. 2004. PMID: 15173622
• A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo.
  Nagai Y, Miyazaki M, Aoki R, Zama T, Inouye S, Hirose K, Iino M, Hagiwara M. Nagai Y, et al. Nat Biotechnol. 2000 Mar;18(3):313-6. doi: 10.1038/73767. Nat Biotechnol. 2000. PMID: 10700148
• Using intrinsically fluorescent proteins for plant cell imaging.
  Dixit R, Cyr R, Gilroy S. Dixit R, et al. Plant J. 2006 Feb;45(4):599-615. doi: 10.1111/j.1365-313X.2006.02658.x. Plant J. 2006. PMID: 16441351 Review.

Cited by

• Single-Chain Lanthanide Luminescence Biosensors for Cell-Based Imaging and Screening of Protein-Protein Interactions.
  Chen T, Pham H, Mohamadi A, Miller LW. Chen T, et al. iScience. 2020 Sep 6;23(9):101533. doi: 10.1016/j.isci.2020.101533. eCollection 2020 Sep 25. iScience. 2020. PMID: 33083762 Free PMC article.
• Networking with AKAPs: context-dependent regulation of anchored enzymes.
  Welch EJ, Jones BW, Scott JD. Welch EJ, et al. Mol Interv. 2010 Apr;10(2):86-97. doi: 10.1124/mi.10.2.6. Mol Interv. 2010. PMID: 20368369 Free PMC article. Review.
• Equilibrium between adenylyl cyclase and phosphodiesterase patterns adrenergic agonist dose-dependent spatiotemporal cAMP/protein kinase A activities in cardiomyocytes.
  De Arcangelis V, Liu S, Zhang D, Soto D, Xiang YK. De Arcangelis V, et al. Mol Pharmacol. 2010 Sep;78(3):340-9. doi: 10.1124/mol.110.064444. Epub 2010 Jun 7. Mol Pharmacol. 2010. PMID: 20530128 Free PMC article.
• Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer.
  Lin W, Mehta S, Zhang J. Lin W, et al. J Biol Chem. 2019 Oct 4;294(40):14814-14822. doi: 10.1074/jbc.REV119.006177. Epub 2019 Aug 21. J Biol Chem. 2019. PMID: 31434714 Free PMC article. Review.
• Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling.
  Kankanamge D, Ratnayake K, Senarath K, Tennakoon M, Harmon E, Karunarathne A. Kankanamge D, et al. Anal Bioanal Chem. 2019 Jul;411(19):4481-4508. doi: 10.1007/s00216-019-01774-6. Epub 2019 Mar 30. Anal Bioanal Chem. 2019. PMID: 30927013 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database