Nanoscale resolution in GFP-based microscopy - PubMed (original) (raw)

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Nanoscale resolution in GFP-based microscopy

Katrin I Willig et al. Nat Methods. 2006 Sep.

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

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