Long-term depression activates transcription of immediate early transcription factor genes: involvement of serum response factor/Elk-1 - PubMed (original) (raw)
Long-term depression activates transcription of immediate early transcription factor genes: involvement of serum response factor/Elk-1
Antje Lindecke et al. Eur J Neurosci. 2006 Jul.
Abstract
Long-term depression (LTD) is one of the paradigms used in vivo or ex vivo for studying memory formation. In order to identify genes with potential relevance for memory formation we used mouse organotypic hippocampal slice cultures in which chemical LTD was induced by applications of 3,5-dihydroxyphenylglycine (DHPG). The induction of chemical LTD was robust, as monitored electrophysiologically. Gene expression analysis after chemical LTD induction was performed using cDNA microarrays containing >7,000 probes. The DHPG-induced expression of immediate early genes (c-fos, junB, egr1 and nr4a1) was subsequently verified by TaqMan polymerase chain reaction. Bioinformatic analysis suggested a common regulator element [serum response factor (SRF)/Elk-1 binding sites] within the promoter region of these genes. Indeed, here we could show a DHPG-dependent binding of SRF at the SRF response element (SRE) site within the promoter region of c-fos and junB. However, SRF binding to egr1 promoter sites was constitutive. The phosphorylation of the ternary complex factor Elk-1 and its localization in the nucleus of hippocampal neurones after DHPG treatment was shown by immunofluorescence using a phosphospecific antibody. We suggest that LTD leads to SRF/Elk-1-regulated gene expression of immediate early transcription factors, which could in turn promote a second broader wave of gene expression.
Similar articles
- FLI1 and EWS-FLI1 function as ternary complex factors and ELK1 and SAP1a function as ternary and quaternary complex factors on the Egr1 promoter serum response elements.
Watson DK, Robinson L, Hodge DR, Kola I, Papas TS, Seth A. Watson DK, et al. Oncogene. 1997 Jan 16;14(2):213-21. doi: 10.1038/sj.onc.1200839. Oncogene. 1997. PMID: 9010223 - The protein phosphatase 1/2A inhibitor okadaic acid increases CREB and Elk-1 phosphorylation and c-fos expression in the rat striatum in vivo.
Choe ES, Parelkar NK, Kim JY, Cho HW, Kang HS, Mao L, Wang JQ. Choe ES, et al. J Neurochem. 2004 Apr;89(2):383-90. doi: 10.1111/j.1471-4159.2003.02334.x. J Neurochem. 2004. PMID: 15056282 - Crystal structure of a ternary SAP-1/SRF/c-fos SRE DNA complex.
Mo Y, Ho W, Johnston K, Marmorstein R. Mo Y, et al. J Mol Biol. 2001 Nov 30;314(3):495-506. doi: 10.1006/jmbi.2001.5138. J Mol Biol. 2001. PMID: 11846562 - Critical Protein-Protein Interactions Determine the Biological Activity of Elk-1, a Master Regulator of Stimulus-Induced Gene Transcription.
Thiel G, Backes TM, Guethlein LA, Rössler OG. Thiel G, et al. Molecules. 2021 Oct 11;26(20):6125. doi: 10.3390/molecules26206125. Molecules. 2021. PMID: 34684708 Free PMC article. Review. - Signal-dependent Elk-1 target genes involved in transcript processing and cell migration.
Kasza A. Kasza A. Biochim Biophys Acta. 2013 Oct;1829(10):1026-33. doi: 10.1016/j.bbagrm.2013.05.004. Epub 2013 May 24. Biochim Biophys Acta. 2013. PMID: 23711433 Review.
Cited by
- Applications of the SR4G Transgenic Zebrafish Line for Biomonitoring of Stress-Disrupting Compounds: A Proof-of-Concept Study.
Nozari A, Do S, Trudeau VL. Nozari A, et al. Front Endocrinol (Lausanne). 2021 Nov 17;12:727777. doi: 10.3389/fendo.2021.727777. eCollection 2021. Front Endocrinol (Lausanne). 2021. PMID: 34867778 Free PMC article. - p38 MAPK pathway-dependent SUMOylation of Elk-1 and phosphorylation of PIAS2 correlate with the downregulation of Elk-1 activity in heat-stressed HeLa cells.
Chowdhury D, Singh A, Gupta A, Tulsawani R, Meena RC, Chakrabarti A. Chowdhury D, et al. Cell Stress Chaperones. 2019 Mar;24(2):393-407. doi: 10.1007/s12192-019-00974-4. Epub 2019 Feb 19. Cell Stress Chaperones. 2019. PMID: 30783905 Free PMC article. - Age-dependent regulation of excitatory synaptic transmission at hippocampal temporoammonic-CA1 synapses by leptin.
McGregor G, Clements L, Farah A, Irving AJ, Harvey J. McGregor G, et al. Neurobiol Aging. 2018 Sep;69:76-93. doi: 10.1016/j.neurobiolaging.2018.05.007. Epub 2018 May 14. Neurobiol Aging. 2018. PMID: 29860205 Free PMC article. - Immediate Early Genes Anchor a Biological Pathway of Proteins Required for Memory Formation, Long-Term Depression and Risk for Schizophrenia.
Marballi KK, Gallitano AL. Marballi KK, et al. Front Behav Neurosci. 2018 Feb 19;12:23. doi: 10.3389/fnbeh.2018.00023. eCollection 2018. Front Behav Neurosci. 2018. PMID: 29520222 Free PMC article. - Roles for Arc in metabotropic glutamate receptor-dependent LTD and synapse elimination: Implications in health and disease.
Wilkerson JR, Albanesi JP, Huber KM. Wilkerson JR, et al. Semin Cell Dev Biol. 2018 May;77:51-62. doi: 10.1016/j.semcdb.2017.09.035. Epub 2017 Oct 14. Semin Cell Dev Biol. 2018. PMID: 28969983 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous