The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing - PubMed (original) (raw)
The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing
M T Huang et al. Mol Cell Biol. 1990 Apr.
Abstract
Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.
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